Method validation of 16 types of structural amino acids using an automated amino acid analyzer

You Shin Shim; Won Jin Yoon; Jaeho Ha; Dongwon Seo; Kwang Won Lee; Woo Young Lee; Kwang Il Kwon; Tae Seok Kang; Jin Ha Lee; Hyun Jeong Kim; Hae Jung Kwak; Sam Pin Lee; So Jung Kim; Won Kap Yun; Junsoo Lee; Jin Bong Hwang

doi:10.1007/s10068-013-0252-0

Method validation of 16 types of structural amino acids using an automated amino acid analyzer

You Shin Shim, Won Jin Yoon, Jaeho Ha, Dongwon Seo, Kwang Won Lee, Woo Young Lee, Kwang Il Kwon, Tae Seok Kang, Jin Ha Lee, Hyun Jeong Kim, Hae Jung Kwak, Sam Pin Lee, So Jung Kim, Won Kap Yun, Junsoo Lee, Jin Bong Hwang

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

An analytical method for the simultaneous determination of 16 types of structural amino acids in infant formula using an automated amino acid analyzer was validated through tests of precision, accuracy and linearity. The automated analysis of the amino acids was performed on an ion-exchange packed column with a visible detector. The certificated reference material (CRM) 1546 from NIST was used as the test sample to determine the precision and accuracy of the analytical method. The regression analyses revealed good correlations [correlation coefficient (r ²)] that were greater than 0.99. The recovery values of the amino acids were ranged from 87.18 to 118.08%. The limits of detection and limits of quantification were less than 0.059 mg/100 g and less than 0.198 mg/100 g, respectively. The intra- and inter-day precisions were less than 14.62% in the CRM sample.

Original language	English
Pages (from-to)	1567-1571
Number of pages	5
Journal	Food Science and Biotechnology
Volume	22
Issue number	6
DOIs	https://doi.org/10.1007/s10068-013-0252-0
Publication status	Published - 2013 Dec

Keywords

amino acids
analytical method
automated amino acid analyzer
post column reaction
validation

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology

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10.1007/s10068-013-0252-0

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Shim, Y. S., Yoon, W. J., Ha, J., Seo, D., Lee, K. W., Lee, W. Y., Kwon, K. I., Kang, T. S., Lee, J. H., Kim, H. J., Kwak, H. J., Lee, S. P., Kim, S. J., Yun, W. K., Lee, J., & Hwang, J. B. (2013). Method validation of 16 types of structural amino acids using an automated amino acid analyzer. Food Science and Biotechnology, 22(6), 1567-1571. https://doi.org/10.1007/s10068-013-0252-0

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title = "Method validation of 16 types of structural amino acids using an automated amino acid analyzer",

abstract = "An analytical method for the simultaneous determination of 16 types of structural amino acids in infant formula using an automated amino acid analyzer was validated through tests of precision, accuracy and linearity. The automated analysis of the amino acids was performed on an ion-exchange packed column with a visible detector. The certificated reference material (CRM) 1546 from NIST was used as the test sample to determine the precision and accuracy of the analytical method. The regression analyses revealed good correlations [correlation coefficient (r 2)] that were greater than 0.99. The recovery values of the amino acids were ranged from 87.18 to 118.08%. The limits of detection and limits of quantification were less than 0.059 mg/100 g and less than 0.198 mg/100 g, respectively. The intra- and inter-day precisions were less than 14.62% in the CRM sample.",

keywords = "amino acids, analytical method, automated amino acid analyzer, post column reaction, validation",

author = "Shim, {You Shin} and Yoon, {Won Jin} and Jaeho Ha and Dongwon Seo and Lee, {Kwang Won} and Lee, {Woo Young} and Kwon, {Kwang Il} and Kang, {Tae Seok} and Lee, {Jin Ha} and Kim, {Hyun Jeong} and Kwak, {Hae Jung} and Lee, {Sam Pin} and Kim, {So Jung} and Yun, {Won Kap} and Junsoo Lee and Hwang, {Jin Bong}",

note = "Funding Information: Acknowledgments This research was supported by a grant (11602KFDA209) from Korea Food and Drug Administration in 2011.",

year = "2013",

month = dec,

doi = "10.1007/s10068-013-0252-0",

language = "English",

volume = "22",

pages = "1567--1571",

journal = "Food Science and Biotechnology",

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T1 - Method validation of 16 types of structural amino acids using an automated amino acid analyzer

AU - Shim, You Shin

AU - Yoon, Won Jin

AU - Ha, Jaeho

AU - Seo, Dongwon

AU - Lee, Kwang Won

AU - Lee, Woo Young

AU - Kwon, Kwang Il

AU - Kang, Tae Seok

AU - Lee, Jin Ha

AU - Kim, Hyun Jeong

AU - Kwak, Hae Jung

AU - Lee, Sam Pin

AU - Kim, So Jung

AU - Yun, Won Kap

AU - Lee, Junsoo

AU - Hwang, Jin Bong

N1 - Funding Information: Acknowledgments This research was supported by a grant (11602KFDA209) from Korea Food and Drug Administration in 2011.

PY - 2013/12

Y1 - 2013/12

N2 - An analytical method for the simultaneous determination of 16 types of structural amino acids in infant formula using an automated amino acid analyzer was validated through tests of precision, accuracy and linearity. The automated analysis of the amino acids was performed on an ion-exchange packed column with a visible detector. The certificated reference material (CRM) 1546 from NIST was used as the test sample to determine the precision and accuracy of the analytical method. The regression analyses revealed good correlations [correlation coefficient (r 2)] that were greater than 0.99. The recovery values of the amino acids were ranged from 87.18 to 118.08%. The limits of detection and limits of quantification were less than 0.059 mg/100 g and less than 0.198 mg/100 g, respectively. The intra- and inter-day precisions were less than 14.62% in the CRM sample.

AB - An analytical method for the simultaneous determination of 16 types of structural amino acids in infant formula using an automated amino acid analyzer was validated through tests of precision, accuracy and linearity. The automated analysis of the amino acids was performed on an ion-exchange packed column with a visible detector. The certificated reference material (CRM) 1546 from NIST was used as the test sample to determine the precision and accuracy of the analytical method. The regression analyses revealed good correlations [correlation coefficient (r 2)] that were greater than 0.99. The recovery values of the amino acids were ranged from 87.18 to 118.08%. The limits of detection and limits of quantification were less than 0.059 mg/100 g and less than 0.198 mg/100 g, respectively. The intra- and inter-day precisions were less than 14.62% in the CRM sample.

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Method validation of 16 types of structural amino acids using an automated amino acid analyzer

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