TY - JOUR
T1 - Microarray analysis of gene expression alteration in human middle ear epithelial cells induced by Asian sand dust
AU - Go, Yoon Young
AU - Park, Moo Kyun
AU - Kwon, Jee Young
AU - Seo, Young Rok
AU - Chae, Sung Won
AU - Song, Jae Jun
N1 - Publisher Copyright:
© 2015 by Korean Society of Otorhinolaryngology-Head and Neck Surgery.
PY - 2015/12
Y1 - 2015/12
N2 - Objectives. The primary aim of this study is to evaluate the gene expression profile of Asian sand dust (ASD)-treated human middle ear epithelial cell (HMEEC) using microarray analysis. Methods. The HMEEC was treated with ASD (400 µg/mL) and total RNA was extracted for microarray analysis. Molecular pathways among differentially expressed genes were further analyzed. For selected genes, the changes in gene expression were confirmed by real-time polymerase chain reaction. Results. A total of 1,274 genes were differentially expressed by ASD. Among them, 1,138 genes were 2 folds up-regulated, whereas 136 genes were 2 folds down-regulated. Up-regulated genes were mainly involved in cellular processes, including apoptosis, cell differentiation, and cell proliferation. Down-regulated genes affected cellular processes, including apoptosis, cell cycle, cell differentiation, and cell proliferation.The 10 genes including ADM, CCL5, EDN1, EGR1, FOS, GHRL, JUN, SOCS3, TNF, and TNFSF10 were identified as main modulators in up-regulated genes. A total of 11 genes including CSF3, DKK1, FOSL1, FST, TERT, MMP13, PTHLH, SPRY2, TGFBR2, THBS1, and TIMP1 acted as main components of pathway associated with 2-fold down regulated genes. Conclusion. We identified the differentially expressed genes in ASD-treated HMEEC. Our work indicates that air pollutant like ASD, may play an important role in the pathogenesis of otitis media.
AB - Objectives. The primary aim of this study is to evaluate the gene expression profile of Asian sand dust (ASD)-treated human middle ear epithelial cell (HMEEC) using microarray analysis. Methods. The HMEEC was treated with ASD (400 µg/mL) and total RNA was extracted for microarray analysis. Molecular pathways among differentially expressed genes were further analyzed. For selected genes, the changes in gene expression were confirmed by real-time polymerase chain reaction. Results. A total of 1,274 genes were differentially expressed by ASD. Among them, 1,138 genes were 2 folds up-regulated, whereas 136 genes were 2 folds down-regulated. Up-regulated genes were mainly involved in cellular processes, including apoptosis, cell differentiation, and cell proliferation. Down-regulated genes affected cellular processes, including apoptosis, cell cycle, cell differentiation, and cell proliferation.The 10 genes including ADM, CCL5, EDN1, EGR1, FOS, GHRL, JUN, SOCS3, TNF, and TNFSF10 were identified as main modulators in up-regulated genes. A total of 11 genes including CSF3, DKK1, FOSL1, FST, TERT, MMP13, PTHLH, SPRY2, TGFBR2, THBS1, and TIMP1 acted as main components of pathway associated with 2-fold down regulated genes. Conclusion. We identified the differentially expressed genes in ASD-treated HMEEC. Our work indicates that air pollutant like ASD, may play an important role in the pathogenesis of otitis media.
KW - Air pollution
KW - Asian sand dust
KW - Microarray analysis
KW - Otitis media
KW - Particulate matter
UR - http://www.scopus.com/inward/record.url?scp=84948176750&partnerID=8YFLogxK
U2 - 10.3342/ceo.2015.8.4.345
DO - 10.3342/ceo.2015.8.4.345
M3 - Article
AN - SCOPUS:84948176750
SN - 1976-8710
VL - 8
SP - 345
EP - 353
JO - Clinical and Experimental Otorhinolaryngology
JF - Clinical and Experimental Otorhinolaryngology
IS - 4
ER -