TY - JOUR
T1 - Microarray analysis of normal cervix, carcinoma in situ, and invasive cervical cancer
T2 - Identification of candidate genes in pathogenesis of invasion in cervical cancer
AU - Song, J. Y.
AU - Lee, J. K.
AU - Lee, N. W.
AU - Jung, H. H.
AU - Kim, S. H.
AU - Lee, K. W.
PY - 2008/9
Y1 - 2008/9
N2 - The objective of this study was to identify genes that are related to pathogenesis of carcinoma in situ (CIS) to invasive cervical cancer with the use of oligonucleotide microarray and reverse transcription-polymerase chain reaction (RT-PCR). Each two cases of normal cervix, CIS, and invasive cervical cancer were investigated with DNA microarray technology. Differential gene expression profiles among them were analyzed. Expression levels of selected genes from the microarray results were confirmed by RT-PCR. The expressions of 15,286 genes were compared and 458 genes were upregulated or downregulated by twofold or more compared with each other group. Among 458 genes, 22 genes were upregulated and 40 genes were downregulated by twofold or more in invasive cervical cancer group compared with CIS group. RT-PCR analysis confirmed upregulation of 18 genes and downregulation of 5 genes in invasive cervical cancer group. RBP1, TFRC, SPP1, SAA1, ARHGAP8, and NDRG1, which were upregulated, and GATA3, PLAGL1, APOD, DUSP1, and CYR61, which were downregulated, were considered as candidate genes associated with invasion of cervical cancer.
AB - The objective of this study was to identify genes that are related to pathogenesis of carcinoma in situ (CIS) to invasive cervical cancer with the use of oligonucleotide microarray and reverse transcription-polymerase chain reaction (RT-PCR). Each two cases of normal cervix, CIS, and invasive cervical cancer were investigated with DNA microarray technology. Differential gene expression profiles among them were analyzed. Expression levels of selected genes from the microarray results were confirmed by RT-PCR. The expressions of 15,286 genes were compared and 458 genes were upregulated or downregulated by twofold or more compared with each other group. Among 458 genes, 22 genes were upregulated and 40 genes were downregulated by twofold or more in invasive cervical cancer group compared with CIS group. RT-PCR analysis confirmed upregulation of 18 genes and downregulation of 5 genes in invasive cervical cancer group. RBP1, TFRC, SPP1, SAA1, ARHGAP8, and NDRG1, which were upregulated, and GATA3, PLAGL1, APOD, DUSP1, and CYR61, which were downregulated, were considered as candidate genes associated with invasion of cervical cancer.
KW - CIS
KW - Candidate genes
KW - Cervical cancer
KW - Gene expression
KW - Microarray
UR - http://www.scopus.com/inward/record.url?scp=44449168056&partnerID=8YFLogxK
U2 - 10.1111/j.1525-1438.2007.01164.x
DO - 10.1111/j.1525-1438.2007.01164.x
M3 - Article
C2 - 18217980
AN - SCOPUS:44449168056
SN - 1048-891X
VL - 18
SP - 1051
EP - 1059
JO - International Journal of Gynecological Cancer
JF - International Journal of Gynecological Cancer
IS - 5
ER -
