Microbial consortia for saccharification of woody biomass and ethanol fermentation

Dayanand Kalyani, Kyung Min Lee, Tae Su Kim, Jinglin Li, Saurabh Sudha Dhiman, Yun Chan Kang, Jung Kul Lee

Research output: Contribution to journalArticlepeer-review

90 Citations (Scopus)


Newly isolated Sistotrema brinkmannii and Agaricus arvensis were co-cultured using rice straw (20 g/L) as a carbon source and yeast extract (10 g/L) as a nitrogen source to produce high levels of cellobiohydrolase (12.6 U/mL), β-glucosidase (21 U/mL), and endoglucanase (16.4 U/mL). The filter paper activity (FPU) of the mixed fungal culture was enhanced (1.61 FPU/mL) compared to that of S. brinkmannii (0.3 FPU/mL) or A. arvensis (0.5 FPU/mL) monoculture. Enzyme loading, substrate concentration, pH, and temperature were optimized by response surface methodology (RSM) to improve the saccharification yield of alkali-pretreated plant biomasses. The highest enzymatic hydrolysis (76.7%) was obtained from Pinus densiflora under the following conditions: crude enzyme loading 22.5 FPU/g-substrate, substrate 3.75%, temperature 35 °C, and pH 5. The enzymatic hydrolyzate of pretreated P. densiflora was used for ethanol production using Saccharomyces cerevisiae, Pichia stipitis, and a co-culture of both the strains. The co-culture of S. cerevisiae and P. stipitis produced 23% more ethanol than that produced by S. cerevisiae alone and 38% more ethanol than that produced by P. stipitis alone. This study shows the potential of exploiting a microbial consortium for the cost-effective production of cellulases for bioethanol processes.

Original languageEnglish
Pages (from-to)815-822
Number of pages8
Publication statusPublished - 2013
Externally publishedYes

Bibliographical note

Funding Information:
This research was supported by the Converging Research Center Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2011-50210 ). This work was also supported by the NRF grant funded by the Korea government (2012M3C5A1053339).


  • Cellulase
  • Ethanol
  • Fungi
  • Microbial consortia
  • Saccharification

ASJC Scopus subject areas

  • General Chemical Engineering
  • Fuel Technology
  • Energy Engineering and Power Technology
  • Organic Chemistry


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