Microbial production of ethanol from acetate by engineered Ralstonia eutropha

Hye Mi Lee; Bo Young Jeon; Min Kyu Oh

doi:10.1007/s12257-016-0197-2

Microbial production of ethanol from acetate by engineered Ralstonia eutropha

Hye Mi Lee, Bo Young Jeon, Min Kyu Oh

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

This study was performed to produce ethanol from acetate using a genetically engineered Ralstonia eutropha. In order to genetically modify R. eutropha H16, phaCAB operon encoding metabolic pathway genes from acetyl-CoA to polyhydroxybutyrate (PHB) was deleted and adhE encoding an alcohol dehydrogenase from Escherichia coli was overexpressed for conversion of acetyl-CoA to ethanol. The resulting strain produced ethanol up to 170 mg/L when cultivated in minimal media supplemented with 5 g/L of acetate as a sole carbon source. Growth and ethanol production were optimized by adjusting nitrogen source (NH₄Cl) content and repetitive feeding of acetate into the bacterial culture, by which the ethanol production was reached to approximately 350 mg/L for 84 h.

Original language	English
Pages (from-to)	402-407
Number of pages	6
Journal	Biotechnology and Bioprocess Engineering
Volume	21
Issue number	3
DOIs	https://doi.org/10.1007/s12257-016-0197-2
Publication status	Published - 2016 Jun 1

Keywords

Ralstonia eutropha
acetate
ethanol
metabolic engineering

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Biomedical Engineering

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10.1007/s12257-016-0197-2

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title = "Microbial production of ethanol from acetate by engineered Ralstonia eutropha",

abstract = "This study was performed to produce ethanol from acetate using a genetically engineered Ralstonia eutropha. In order to genetically modify R. eutropha H16, phaCAB operon encoding metabolic pathway genes from acetyl-CoA to polyhydroxybutyrate (PHB) was deleted and adhE encoding an alcohol dehydrogenase from Escherichia coli was overexpressed for conversion of acetyl-CoA to ethanol. The resulting strain produced ethanol up to 170 mg/L when cultivated in minimal media supplemented with 5 g/L of acetate as a sole carbon source. Growth and ethanol production were optimized by adjusting nitrogen source (NH4Cl) content and repetitive feeding of acetate into the bacterial culture, by which the ethanol production was reached to approximately 350 mg/L for 84 h.",

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AB - This study was performed to produce ethanol from acetate using a genetically engineered Ralstonia eutropha. In order to genetically modify R. eutropha H16, phaCAB operon encoding metabolic pathway genes from acetyl-CoA to polyhydroxybutyrate (PHB) was deleted and adhE encoding an alcohol dehydrogenase from Escherichia coli was overexpressed for conversion of acetyl-CoA to ethanol. The resulting strain produced ethanol up to 170 mg/L when cultivated in minimal media supplemented with 5 g/L of acetate as a sole carbon source. Growth and ethanol production were optimized by adjusting nitrogen source (NH4Cl) content and repetitive feeding of acetate into the bacterial culture, by which the ethanol production was reached to approximately 350 mg/L for 84 h.

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Microbial production of ethanol from acetate by engineered Ralstonia eutropha

Abstract

Keywords

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