Microbial production of ethanol from acetate by engineered Ralstonia eutropha

Hye Mi Lee, Bo Young Jeon, Min Kyu Oh

    Research output: Contribution to journalArticlepeer-review

    37 Citations (Scopus)

    Abstract

    This study was performed to produce ethanol from acetate using a genetically engineered Ralstonia eutropha. In order to genetically modify R. eutropha H16, phaCAB operon encoding metabolic pathway genes from acetyl-CoA to polyhydroxybutyrate (PHB) was deleted and adhE encoding an alcohol dehydrogenase from Escherichia coli was overexpressed for conversion of acetyl-CoA to ethanol. The resulting strain produced ethanol up to 170 mg/L when cultivated in minimal media supplemented with 5 g/L of acetate as a sole carbon source. Growth and ethanol production were optimized by adjusting nitrogen source (NH4Cl) content and repetitive feeding of acetate into the bacterial culture, by which the ethanol production was reached to approximately 350 mg/L for 84 h.

    Original languageEnglish
    Pages (from-to)402-407
    Number of pages6
    JournalBiotechnology and Bioprocess Engineering
    Volume21
    Issue number3
    DOIs
    Publication statusPublished - 2016 Jun 1

    Bibliographical note

    Publisher Copyright:
    © 2016, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.

    Keywords

    • Ralstonia eutropha
    • acetate
    • ethanol
    • metabolic engineering

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology
    • Biomedical Engineering

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