TY - GEN
T1 - Microfluidic assay to study transendothelial migration of human leukocyte
AU - Han, Sewoon
AU - Yan, Ji Jing
AU - Shin, Yoojin
AU - Kamm, Roger D.
AU - Kim, Young Joon
AU - Chung, Seok
PY - 2011
Y1 - 2011
N2 - Most of the study on transendothelial migration has been conducted with 2D in vitro assays, which are not appropriate for high-resolution imaging of cell-cell interaction, quantification of the cell migration ability in time-dependency, and investigation of 3D cell migration. For these reasons, the complex transendothelial migration cascades have hardly ever discovered. To overcome these drawbacks of several established in vitro essays, here, we introduce the hydrogel scaffold embedded microfluidic platform. In this study, we have demonstrated the transendothelial migration of human leukocytes interacting with hMVECs monolayer with the precisely regulated chemotactic gradient of inflammatory mediators.
AB - Most of the study on transendothelial migration has been conducted with 2D in vitro assays, which are not appropriate for high-resolution imaging of cell-cell interaction, quantification of the cell migration ability in time-dependency, and investigation of 3D cell migration. For these reasons, the complex transendothelial migration cascades have hardly ever discovered. To overcome these drawbacks of several established in vitro essays, here, we introduce the hydrogel scaffold embedded microfluidic platform. In this study, we have demonstrated the transendothelial migration of human leukocytes interacting with hMVECs monolayer with the precisely regulated chemotactic gradient of inflammatory mediators.
KW - 3D cell migration
KW - Extracellular matrix (ECM)
KW - Microfluidics
KW - Transendothelial migration
UR - http://www.scopus.com/inward/record.url?scp=84883746428&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883746428&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883746428
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 822
EP - 824
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -