Microfluidic assay to study transendothelial migration of human leukocyte

Sewoon Han, Ji Jing Yan, Yoojin Shin, Roger D. Kamm, Young Joon Kim, Seok Chung

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Most of the study on transendothelial migration has been conducted with 2D in vitro assays, which are not appropriate for high-resolution imaging of cell-cell interaction, quantification of the cell migration ability in time-dependency, and investigation of 3D cell migration. For these reasons, the complex transendothelial migration cascades have hardly ever discovered. To overcome these drawbacks of several established in vitro essays, here, we introduce the hydrogel scaffold embedded microfluidic platform. In this study, we have demonstrated the transendothelial migration of human leukocytes interacting with hMVECs monolayer with the precisely regulated chemotactic gradient of inflammatory mediators.

    Original languageEnglish
    Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
    Pages822-824
    Number of pages3
    Publication statusPublished - 2011
    Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
    Duration: 2011 Oct 22011 Oct 6

    Publication series

    Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
    Volume2

    Other

    Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
    Country/TerritoryUnited States
    CitySeattle, WA
    Period11/10/211/10/6

    Keywords

    • 3D cell migration
    • Extracellular matrix (ECM)
    • Microfluidics
    • Transendothelial migration

    ASJC Scopus subject areas

    • Control and Systems Engineering

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