MICROFLUIDIC CELL SHEARING ENABLES HIGHLY EFFECTIVE MACROMOLECULE INTRACELLULAR DELIVERY

Chan Kwon, Geoum Young Kang, Aram Chung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A novel microfluidic cell stretching platform using viscoelastic fluids is presented, enabling highly effective intracellular delivery of external biomolecules into various cell types. The cell suspended in methylcellulose (MC) solution is injected into the platform, where cells rapidly pass through a single constriction with a dimension slightly smaller than the cell diameter. Because of the MC solution, a high shear force is applied to the cells, effectively generating transient cell membrane discontinuities. With this approach, a high delivery efficiency (>97%), high throughput (>106 cells/min) and nearly clogging-free of various macromolecules into different immune cells were demonstrated for cancer immunotherapy applications.

Original languageEnglish
Title of host publicationMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages649-650
Number of pages2
ISBN (Electronic)9781733419031
Publication statusPublished - 2021
Event25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States
Duration: 2021 Oct 102021 Oct 14

Publication series

NameMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/TerritoryUnited States
CityPalm Springs, Virtual
Period21/10/1021/10/14

Bibliographical note

Funding Information:
This work was supported by the Samsung Research Funding and Incubation Center for Future Technology (Grant No. SRFCIT1802-03) and the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (No. 2021R1A2C2006224).

Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

Keywords

  • Cancer immunotherapy
  • Intracellular delivery
  • Microfluidic cell shearing
  • mRNA delivery

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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