MICROFLUIDIC CELL SHEARING ENABLES HIGHLY EFFECTIVE MACROMOLECULE INTRACELLULAR DELIVERY

Chan Kwon; Geoum Young Kang; Aram Chung

MICROFLUIDIC CELL SHEARING ENABLES HIGHLY EFFECTIVE MACROMOLECULE INTRACELLULAR DELIVERY

Chan Kwon, Geoum Young Kang, Aram Chung

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A novel microfluidic cell stretching platform using viscoelastic fluids is presented, enabling highly effective intracellular delivery of external biomolecules into various cell types. The cell suspended in methylcellulose (MC) solution is injected into the platform, where cells rapidly pass through a single constriction with a dimension slightly smaller than the cell diameter. Because of the MC solution, a high shear force is applied to the cells, effectively generating transient cell membrane discontinuities. With this approach, a high delivery efficiency (>97%), high throughput (>10⁶ cells/min) and nearly clogging-free of various macromolecules into different immune cells were demonstrated for cancer immunotherapy applications.

Original language	English
Title of host publication	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	649-650
Number of pages	2
ISBN (Electronic)	9781733419031
Publication status	Published - 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States Duration: 2021 Oct 10 → 2021 Oct 14

Publication series

Name	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/Territory	United States
City	Palm Springs, Virtual
Period	21/10/10 → 21/10/14

Bibliographical note

Funding Information:
This work was supported by the Samsung Research Funding and Incubation Center for Future Technology (Grant No. SRFCIT1802-03) and the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (No. 2021R1A2C2006224).

Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

Keywords

Cancer immunotherapy
Intracellular delivery
Microfluidic cell shearing
mRNA delivery

ASJC Scopus subject areas

Bioengineering
Chemical Engineering (miscellaneous)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

Kwon, C., Kang, G. Y., & Chung, A. (2021). MICROFLUIDIC CELL SHEARING ENABLES HIGHLY EFFECTIVE MACROMOLECULE INTRACELLULAR DELIVERY. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 649-650). (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

MICROFLUIDIC CELL SHEARING ENABLES HIGHLY EFFECTIVE MACROMOLECULE INTRACELLULAR DELIVERY. / Kwon, Chan; Kang, Geoum Young; Chung, Aram.
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. p. 649-650 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Kwon, C, Kang, GY & Chung, A 2021, MICROFLUIDIC CELL SHEARING ENABLES HIGHLY EFFECTIVE MACROMOLECULE INTRACELLULAR DELIVERY. in MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 649-650, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, Palm Springs, Virtual, United States, 21/10/10.

Kwon C, Kang GY, Chung A. MICROFLUIDIC CELL SHEARING ENABLES HIGHLY EFFECTIVE MACROMOLECULE INTRACELLULAR DELIVERY. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2021. p. 649-650. (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Kwon, Chan ; Kang, Geoum Young ; Chung, Aram. / MICROFLUIDIC CELL SHEARING ENABLES HIGHLY EFFECTIVE MACROMOLECULE INTRACELLULAR DELIVERY. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. pp. 649-650 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

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abstract = "A novel microfluidic cell stretching platform using viscoelastic fluids is presented, enabling highly effective intracellular delivery of external biomolecules into various cell types. The cell suspended in methylcellulose (MC) solution is injected into the platform, where cells rapidly pass through a single constriction with a dimension slightly smaller than the cell diameter. Because of the MC solution, a high shear force is applied to the cells, effectively generating transient cell membrane discontinuities. With this approach, a high delivery efficiency (>97%), high throughput (>106 cells/min) and nearly clogging-free of various macromolecules into different immune cells were demonstrated for cancer immunotherapy applications.",

keywords = "Cancer immunotherapy, Intracellular delivery, Microfluidic cell shearing, mRNA delivery",

author = "Chan Kwon and Kang, {Geoum Young} and Aram Chung",

note = "Funding Information: This work was supported by the Samsung Research Funding and Incubation Center for Future Technology (Grant No. SRFCIT1802-03) and the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (No. 2021R1A2C2006224). Publisher Copyright: {\textcopyright} 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

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language = "English",

series = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

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AU - Kang, Geoum Young

AU - Chung, Aram

N1 - Funding Information: This work was supported by the Samsung Research Funding and Incubation Center for Future Technology (Grant No. SRFCIT1802-03) and the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (No. 2021R1A2C2006224). Publisher Copyright: © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

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N2 - A novel microfluidic cell stretching platform using viscoelastic fluids is presented, enabling highly effective intracellular delivery of external biomolecules into various cell types. The cell suspended in methylcellulose (MC) solution is injected into the platform, where cells rapidly pass through a single constriction with a dimension slightly smaller than the cell diameter. Because of the MC solution, a high shear force is applied to the cells, effectively generating transient cell membrane discontinuities. With this approach, a high delivery efficiency (>97%), high throughput (>106 cells/min) and nearly clogging-free of various macromolecules into different immune cells were demonstrated for cancer immunotherapy applications.

AB - A novel microfluidic cell stretching platform using viscoelastic fluids is presented, enabling highly effective intracellular delivery of external biomolecules into various cell types. The cell suspended in methylcellulose (MC) solution is injected into the platform, where cells rapidly pass through a single constriction with a dimension slightly smaller than the cell diameter. Because of the MC solution, a high shear force is applied to the cells, effectively generating transient cell membrane discontinuities. With this approach, a high delivery efficiency (>97%), high throughput (>106 cells/min) and nearly clogging-free of various macromolecules into different immune cells were demonstrated for cancer immunotherapy applications.

KW - Cancer immunotherapy

KW - Intracellular delivery

KW - Microfluidic cell shearing

KW - mRNA delivery

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AN - SCOPUS:85136921074

T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 649

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BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

MICROFLUIDIC CELL SHEARING ENABLES HIGHLY EFFECTIVE MACROMOLECULE INTRACELLULAR DELIVERY

Abstract

Publication series

Conference

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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Cite this