The precise manipulation of the neural stem cell (NSC)-derived neural differentiation is still challenging, and there is a technological barrier to regulate the axonal regeneration in a controlled manner. Here, we developed a microfluidic chip integrated with a microelectrode array as an axonal guidance platform. The microfluidic electrode array chip consisted of two compartments and a bridge microchannel that could isolate and guide the axons. We demonstrated that the NSCs were largely differentiated into neural cells as the electric field was applied to the microfluidic electrode array chip. We also confirmed the synergistic effects of the electrical stimulation (ES) and neurotrophic factor (NF) on axonal outgrowth. This microfluidic electrode array chip can serve as a central nervous system (CNS) model for axonal injury and regeneration. Therefore, it could be a potentially powerful tool for an in vitro model of the axonal regeneration.
Bibliographical noteFunding Information:
This work was supported by the National Research Foundation of Korea grant funded by the Ministry of Science and ICT (Grant number 2022R1A2C2003724, 2019M3A9H2032547, 2016R1A6A1A03012845, and 2021M3E5D9021368). This research was also supported by Korean Fund for Regenerative Medicine funded by Ministry of Science and ICT, and Ministry of Health and Welfare (Grant number RS-2022-00070316), Republic of Korea.
© 2022 The Royal Society of Chemistry
ASJC Scopus subject areas
- Biomedical Engineering