Abstract
We report a novel microfluidic vector-less intracellular delivery platform that effectively internalizes various nanomaterials into different immune cell lines without the use of external forces. The cells suspended in Methylcellulose solution are designed to pass a single constriction where high shear stresses and pressure gradients are imposed to deform cells. Due to cell shearing, transient nanopores on cell membranes are effectively generated, and target nanomaterials from the surrounding medium can be easily diffused into the cell cytosol. Using this approach, highly efficient, high-throughput, and rapid delivery of macromolecules into different cell lines was accomplished while maintaining high cell viability.
| Original language | English |
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| Title of host publication | MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1033-1034 |
| Number of pages | 2 |
| ISBN (Electronic) | 9781733419017 |
| Publication status | Published - 2020 |
| Event | 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online Duration: 2020 Oct 4 → 2020 Oct 9 |
Publication series
| Name | MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
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Conference
| Conference | 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 |
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| City | Virtual, Online |
| Period | 20/10/4 → 20/10/9 |
Bibliographical note
Funding Information:This study was supported by the National Research Foundation of Korea (NRF), funded by the Ministry of Education(NRF-2018R1D1A1B07045538).
Publisher Copyright:
© 2020 CBMS-0001
Keywords
- Cell Stretching
- Fluid Cell Shearing
- Intracellular Delivery
- Nanomaterial Delivery
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering
- General Chemistry
- Control and Systems Engineering