TY - GEN
T1 - Microfluidic intracellular delivery via fluid cell shearing
AU - Kang, Geoum Young
AU - Kwon, Chan
AU - Chung, Aram
N1 - Funding Information:
This study was supported by the National Research Foundation of Korea (NRF), funded by the Ministry of Education(NRF-2018R1D1A1B07045538).
Publisher Copyright:
© 2020 CBMS-0001
PY - 2020
Y1 - 2020
N2 - We report a novel microfluidic vector-less intracellular delivery platform that effectively internalizes various nanomaterials into different immune cell lines without the use of external forces. The cells suspended in Methylcellulose solution are designed to pass a single constriction where high shear stresses and pressure gradients are imposed to deform cells. Due to cell shearing, transient nanopores on cell membranes are effectively generated, and target nanomaterials from the surrounding medium can be easily diffused into the cell cytosol. Using this approach, highly efficient, high-throughput, and rapid delivery of macromolecules into different cell lines was accomplished while maintaining high cell viability.
AB - We report a novel microfluidic vector-less intracellular delivery platform that effectively internalizes various nanomaterials into different immune cell lines without the use of external forces. The cells suspended in Methylcellulose solution are designed to pass a single constriction where high shear stresses and pressure gradients are imposed to deform cells. Due to cell shearing, transient nanopores on cell membranes are effectively generated, and target nanomaterials from the surrounding medium can be easily diffused into the cell cytosol. Using this approach, highly efficient, high-throughput, and rapid delivery of macromolecules into different cell lines was accomplished while maintaining high cell viability.
KW - Cell Stretching
KW - Fluid Cell Shearing
KW - Intracellular Delivery
KW - Nanomaterial Delivery
UR - http://www.scopus.com/inward/record.url?scp=85098275795&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85098275795
T3 - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1033
EP - 1034
BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
Y2 - 4 October 2020 through 9 October 2020
ER -