Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration

Hyunho Kim; Sung Hee Hong; Hyo Eun Jeong; Sewoon Han; Jinchul Ahn; Jin A. Kim; Ji Hun Yang; Hyun Jeong Oh; Seok Chung; Sang Eun Lee

doi:10.1038/s41598-022-15305-4

Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration

Hyunho Kim, Sung Hee Hong, Hyo Eun Jeong, Sewoon Han, Jinchul Ahn, Jin A. Kim, Ji Hun Yang, Hyun Jeong Oh, Seok Chung, Sang Eun Lee

School of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

The protozoan parasite Toxoplasma gondii (T. gondii) causes one of the most common human zoonotic diseases and infects approximately one-third of the global population. T. gondii infects nearly every cell type and causes severe symptoms in susceptible populations. In previous laboratory animal studies, T. gondii movement and transmission were not analyzed in real time. In a three-dimensional (3D) microfluidic assay, we successfully supported the complex lytic cycle of T. gondii in situ by generating a stable microvasculature. The physiology of the T. gondii-infected microvasculature was monitored in order to investigate the growth, paracellular and transcellular migration, and transmission of T. gondii, as well as the efficacy of T. gondii drugs.

Original language	English
Article number	11449
Journal	Scientific reports
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41598-022-15305-4
Publication status	Published - 2022 Dec

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10.1038/s41598-022-15305-4

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title = "Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration",

abstract = "The protozoan parasite Toxoplasma gondii (T. gondii) causes one of the most common human zoonotic diseases and infects approximately one-third of the global population. T. gondii infects nearly every cell type and causes severe symptoms in susceptible populations. In previous laboratory animal studies, T. gondii movement and transmission were not analyzed in real time. In a three-dimensional (3D) microfluidic assay, we successfully supported the complex lytic cycle of T. gondii in situ by generating a stable microvasculature. The physiology of the T. gondii-infected microvasculature was monitored in order to investigate the growth, paracellular and transcellular migration, and transmission of T. gondii, as well as the efficacy of T. gondii drugs.",

author = "Hyunho Kim and Hong, {Sung Hee} and Jeong, {Hyo Eun} and Sewoon Han and Jinchul Ahn and Kim, {Jin A.} and Yang, {Ji Hun} and Oh, {Hyun Jeong} and Seok Chung and Lee, {Sang Eun}",

note = "Funding Information: This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government. (MSIT) (No. 2020R1A2B5B03002005) and the Korea Evaluation Institute of Industrial Technology (KEIT) grant funded by the Korean government (MSIT) (No. 20009125). H.J. Oh was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. 2020R1C1C1011255) and a Korea University Grant. Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

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doi = "10.1038/s41598-022-15305-4",

language = "English",

volume = "12",

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AU - Kim, Hyunho

AU - Hong, Sung Hee

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AU - Ahn, Jinchul

AU - Kim, Jin A.

AU - Yang, Ji Hun

AU - Oh, Hyun Jeong

AU - Chung, Seok

AU - Lee, Sang Eun

N1 - Funding Information: This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government. (MSIT) (No. 2020R1A2B5B03002005) and the Korea Evaluation Institute of Industrial Technology (KEIT) grant funded by the Korean government (MSIT) (No. 20009125). H.J. Oh was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. 2020R1C1C1011255) and a Korea University Grant. Publisher Copyright: © 2022, The Author(s).

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AB - The protozoan parasite Toxoplasma gondii (T. gondii) causes one of the most common human zoonotic diseases and infects approximately one-third of the global population. T. gondii infects nearly every cell type and causes severe symptoms in susceptible populations. In previous laboratory animal studies, T. gondii movement and transmission were not analyzed in real time. In a three-dimensional (3D) microfluidic assay, we successfully supported the complex lytic cycle of T. gondii in situ by generating a stable microvasculature. The physiology of the T. gondii-infected microvasculature was monitored in order to investigate the growth, paracellular and transcellular migration, and transmission of T. gondii, as well as the efficacy of T. gondii drugs.

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Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration

Abstract

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