Microfluidic perfusion bioreactor for optimization of microalgal lipid productivity

Sang Min Paik; Sang Jun Sim; Noo Li Jeon

doi:10.1016/j.biortech.2017.02.050

Microfluidic perfusion bioreactor for optimization of microalgal lipid productivity

Sang Min Paik
, Sang Jun Sim
, Noo Li Jeon^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Nutrient deprivation in a batch process induces microbes to produce secondary metabolites while drastically constraining cellular growth. A microfluidic continuous perfusion system was designed and tested to culture microalgae, Chlamydomonas reinhardtii, under constant nutrient concentration slightly lower than normal condition. When cultured in 7.5%/7.5% of NH₄ ⁺/PO₄ ² ⁻, C. reinhardtii showed a 2.4-fold increase in TAG production with a 3.5-fold increase in biomass compared to level obtained under an only NH₄ ⁺ depleted condition. The microfluidic continuous perfusion bioreactor with steady continuous nutrient flow can be used to optimize conditions for enhancing secondary metabolite production and increasing microbial biomass.

Original language	English
Pages (from-to)	433-437
Number of pages	5
Journal	Bioresource Technology
Volume	233
DOIs	https://doi.org/10.1016/j.biortech.2017.02.050
Publication status	Published - 2017

Keywords

Continuous flow
Microalgae
Microfluidic perfusion bioreactor
Nutrient gradient
Triacylglycerol

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Waste Management and Disposal

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title = "Microfluidic perfusion bioreactor for optimization of microalgal lipid productivity",

abstract = "Nutrient deprivation in a batch process induces microbes to produce secondary metabolites while drastically constraining cellular growth. A microfluidic continuous perfusion system was designed and tested to culture microalgae, Chlamydomonas reinhardtii, under constant nutrient concentration slightly lower than normal condition. When cultured in 7.5\%/7.5\% of NH4 +/PO4 2 −, C. reinhardtii showed a 2.4-fold increase in TAG production with a 3.5-fold increase in biomass compared to level obtained under an only NH4 + depleted condition. The microfluidic continuous perfusion bioreactor with steady continuous nutrient flow can be used to optimize conditions for enhancing secondary metabolite production and increasing microbial biomass.",

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journal = "Bioresource Technology",

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T1 - Microfluidic perfusion bioreactor for optimization of microalgal lipid productivity

AU - Paik, Sang Min

AU - Sim, Sang Jun

AU - Jeon, Noo Li

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AB - Nutrient deprivation in a batch process induces microbes to produce secondary metabolites while drastically constraining cellular growth. A microfluidic continuous perfusion system was designed and tested to culture microalgae, Chlamydomonas reinhardtii, under constant nutrient concentration slightly lower than normal condition. When cultured in 7.5%/7.5% of NH4 +/PO4 2 −, C. reinhardtii showed a 2.4-fold increase in TAG production with a 3.5-fold increase in biomass compared to level obtained under an only NH4 + depleted condition. The microfluidic continuous perfusion bioreactor with steady continuous nutrient flow can be used to optimize conditions for enhancing secondary metabolite production and increasing microbial biomass.

KW - Continuous flow

KW - Microalgae

KW - Microfluidic perfusion bioreactor

KW - Nutrient gradient

KW - Triacylglycerol

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SN - 0960-8524

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SP - 433

EP - 437

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Microfluidic perfusion bioreactor for optimization of microalgal lipid productivity

Abstract

Keywords

ASJC Scopus subject areas

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