Microfluidic platform to study three dimensional cell migration & capillary morphogenesis

S. Chung; R. Sudo; I. Zervantonakis; T. Rimchala; P. J. Mack; C. R. Wan; V. Vickerman; R. D. Kamm

Microfluidic platform to study three dimensional cell migration & capillary morphogenesis

S. Chung, R. Sudo, I. Zervantonakis, T. Rimchala, P. J. Mack, C. R. Wan, V. Vickerman, R. D. Kamm

Research output: Contribution to conference › Paper › peer-review

Abstract

Here we present a new microfluidic platform that allows for the simultaneous imaging of cells migrating through a 3D matrix, production of a stable, concentration gradient of chemoattractant, and side-by-side comparisons of control and test conditions to normalize for sample-to-sample variability. We demonstrate the system here in a study of the migration of two tumor cell lines and the sprouting of endothelial cells into the matrix from a monolayer. We also show results using the same system to investigate the transmigration of a tumor cell across an endothelial monolayer. These experiments demonstrate the wide range of capabilities of the new system.

Original language	English
Pages	24-26
Number of pages	3
Publication status	Published - 2008
Externally published	Yes
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 2008 Oct 12 → 2008 Oct 16

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	08/10/12 → 08/10/16

Keywords

Capillary morphogenesis
Cell migration
Microfluidic

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Microfluidic platform to study three dimensional cell migration & capillary morphogenesis. / Chung, S.; Sudo, R.; Zervantonakis, I. et al.
2008. 24-26 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

Chung, S, Sudo, R, Zervantonakis, I, Rimchala, T, Mack, PJ, Wan, CR, Vickerman, V & Kamm, RD 2008, 'Microfluidic platform to study three dimensional cell migration & capillary morphogenesis', Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States, 08/10/12 - 08/10/16 pp. 24-26.

@conference{48f7fc002f124533bad268d6bd21d745,

title = "Microfluidic platform to study three dimensional cell migration & capillary morphogenesis",

abstract = "Here we present a new microfluidic platform that allows for the simultaneous imaging of cells migrating through a 3D matrix, production of a stable, concentration gradient of chemoattractant, and side-by-side comparisons of control and test conditions to normalize for sample-to-sample variability. We demonstrate the system here in a study of the migration of two tumor cell lines and the sprouting of endothelial cells into the matrix from a monolayer. We also show results using the same system to investigate the transmigration of a tumor cell across an endothelial monolayer. These experiments demonstrate the wide range of capabilities of the new system.",

keywords = "Capillary morphogenesis, Cell migration, Microfluidic",

author = "S. Chung and R. Sudo and I. Zervantonakis and T. Rimchala and Mack, {P. J.} and Wan, {C. R.} and V. Vickerman and Kamm, {R. D.}",

year = "2008",

language = "English",

pages = "24--26",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

}

TY - CONF

T1 - Microfluidic platform to study three dimensional cell migration & capillary morphogenesis

AU - Chung, S.

AU - Sudo, R.

AU - Zervantonakis, I.

AU - Rimchala, T.

AU - Mack, P. J.

AU - Wan, C. R.

AU - Vickerman, V.

AU - Kamm, R. D.

PY - 2008

Y1 - 2008

N2 - Here we present a new microfluidic platform that allows for the simultaneous imaging of cells migrating through a 3D matrix, production of a stable, concentration gradient of chemoattractant, and side-by-side comparisons of control and test conditions to normalize for sample-to-sample variability. We demonstrate the system here in a study of the migration of two tumor cell lines and the sprouting of endothelial cells into the matrix from a monolayer. We also show results using the same system to investigate the transmigration of a tumor cell across an endothelial monolayer. These experiments demonstrate the wide range of capabilities of the new system.

AB - Here we present a new microfluidic platform that allows for the simultaneous imaging of cells migrating through a 3D matrix, production of a stable, concentration gradient of chemoattractant, and side-by-side comparisons of control and test conditions to normalize for sample-to-sample variability. We demonstrate the system here in a study of the migration of two tumor cell lines and the sprouting of endothelial cells into the matrix from a monolayer. We also show results using the same system to investigate the transmigration of a tumor cell across an endothelial monolayer. These experiments demonstrate the wide range of capabilities of the new system.

KW - Capillary morphogenesis

KW - Cell migration

KW - Microfluidic

UR - http://www.scopus.com/inward/record.url?scp=84902478658&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902478658

SP - 24

EP - 26

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Microfluidic platform to study three dimensional cell migration & capillary morphogenesis

Abstract

Other

Keywords

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this