Microfluidic Potentiometric Cytometry for Size-Selective Micro Dispersion Analysis

Bongjoon Kim, Wenliang Yao, Jong Won Rhie, Honggu Chun

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Impedance-based microfluidic cytometry has proven its potential for applications in point-of-care tests and clinical diagnosis. In this work, we have developed a three-electrode microfluidic potentiometric cytometry system for robust cell size and concentration measurement. A pair of planar electrodes applies a constant voltage drop as a sensing electrode detects the potential changes caused by particle translocation events through a microchannel. The microchannel geometry was optimized based on numerical simulation to provide signal amplitudes of particles independent of cross-sectional position as well as buffer conductivity. Additionally, the signal amplitude shows non-linear characteristics with respect to particle diameter, enabling stem cell selective counting without further signal processing. Experimental results from measurements of the concentration of polystyrene particles as well as stem cells in a stromal vascular fraction (SVF) showed good agreement with manual hemocytometry (error < 2.1%). The developed system is applicable to point-of-care cell, bacteria, and microparticle analyses for global health and environmental monitoring.

Original languageEnglish
Pages (from-to)471-479
Number of pages9
JournalBiochip Journal
Volume16
Issue number4
DOIs
Publication statusPublished - 2022 Dec

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) (NRF-2020R1A2C3010322, NRF-2018M3A9D7079485) and the ministry of SMEs and Startups(SMEs) (S2689276).

Publisher Copyright:
© 2022, The Korean BioChip Society.

Keywords

  • Automated cell counter
  • Coulter counter
  • Microfluidic cytometry
  • Potentiometer
  • Stem cell counting

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

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