Microfluidic spinning of grooved microfiber for guided neuronal cell culture using surface tension mediated grooved round channel

Gi Seok Jeong, Sang Hoon Lee

    Research output: Contribution to journalArticlepeer-review

    17 Citations (Scopus)

    Abstract

    Microfibers with groove structure enable the guided culture of diverse cells. For the fabrication of grooved microfibers, microchannel with a few microscale groove structure needs to be fabricated using complicated photolithography processes. In this study, a novel method to fabricate a round channel embedded microfluidic device for generating continuous grooved microfibers is introduced. The surface tension of PDMS prepolymer was used to construct the round channels and groove patterned structures in the microfluidic device. The self-organized multidroplet structures were utilized to engrave the groove on the microfiber without any complex procedure. Using the device, the groove patterned (ca.10 μm of pattern size) microfiber was successfully fabricated. Microfibers were continuously produced without clogging and the diameters of the grooved microfibers were varied with the flow rate. For the test of usability for cell alignment, fibers were coated with laminin and the neuro-progenitor cells from prenatal rat were seeded on the prepared grooved fibers and cultured. The guided growth of neuronal cells on the fiber was observed through the immunostaining with neurofilament.

    Original languageEnglish
    Pages (from-to)291-296
    Number of pages6
    JournalTissue Engineering and Regenerative Medicine
    Volume11
    Issue number4
    DOIs
    Publication statusPublished - 2014 Aug 1

    Bibliographical note

    Publisher Copyright:
    © 2014, The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media Dordrecht.

    Keywords

    • Grooved microfiber
    • PDMS droplet
    • Round channel
    • neuronal cell guiding
    • surface tension

    ASJC Scopus subject areas

    • Medicine (miscellaneous)
    • Biomedical Engineering

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