Microfluidic sputum homogenizer driven by water-head pressure

Korakot Boonyaphon; Zhenglin Li; Geunyong Kim; Chae Seung Lim; Sung Jin Kim

doi:10.1016/j.snb.2018.09.041

Microfluidic sputum homogenizer driven by water-head pressure

Korakot Boonyaphon, Zhenglin Li, Geunyong Kim, Chae Seung Lim, Sung Jin Kim

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Homogenizing organism distribution in sputum samples is an initial, yet crucial step to diagnose pulmonary diseases with the samples. Although a homogenization platform for a diagnostic system needs to be system-compatible and efficient, it is rarely implemented. Here, we present a pulsatile micromixer for sputum homogenization that is efficient and does not use dynamic external controllers. The device consists of three oscillator and one mixer units. The oscillator unit autonomously converts a constant input of water-head pressure to a pulsatile pressure, and the mixer unit utilizes this pressure to homogeneously mix two solutions. The mixing performance is analyzed with different oscillation frequencies and width of mixer membranes. The device implemented homogeneous micromixing with a 9 Hz pulse for a flow rate up to 350 μL/min and approximately 200 μL/min for 1 cP and 10 cP viscosity solutions, respectively. Importantly, the device demonstrated successful homogenization of sputum samples with cell viabilities similar to those of the standard method for the flow rate up to 100 μL/min, thereby exhibiting biocompatibility with clinical samples. Our device is potentially useful for various lab-on-a-chip applications that require the compatibility of system integration without sacrificing mixing efficiency.

Original language	English
Pages (from-to)	431-436
Number of pages	6
Journal	Sensors and Actuators, B: Chemical
Volume	277
DOIs	https://doi.org/10.1016/j.snb.2018.09.041
Publication status	Published - 2018 Dec 20

Bibliographical note

Funding Information:
The study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) ( NRF-2016R1A5A1010148 , 2017R1A2B4005955 ) and was authored as part of Konkuk University’s research support program for faculty on sabbatical leave in 2018.

Publisher Copyright:
© 2018 Elsevier B.V.

Keywords

High-viscosity mixing
Micromixer
Pulsatile flow
Sputum homogenization

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2018.09.041

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title = "Microfluidic sputum homogenizer driven by water-head pressure",

abstract = "Homogenizing organism distribution in sputum samples is an initial, yet crucial step to diagnose pulmonary diseases with the samples. Although a homogenization platform for a diagnostic system needs to be system-compatible and efficient, it is rarely implemented. Here, we present a pulsatile micromixer for sputum homogenization that is efficient and does not use dynamic external controllers. The device consists of three oscillator and one mixer units. The oscillator unit autonomously converts a constant input of water-head pressure to a pulsatile pressure, and the mixer unit utilizes this pressure to homogeneously mix two solutions. The mixing performance is analyzed with different oscillation frequencies and width of mixer membranes. The device implemented homogeneous micromixing with a 9 Hz pulse for a flow rate up to 350 μL/min and approximately 200 μL/min for 1 cP and 10 cP viscosity solutions, respectively. Importantly, the device demonstrated successful homogenization of sputum samples with cell viabilities similar to those of the standard method for the flow rate up to 100 μL/min, thereby exhibiting biocompatibility with clinical samples. Our device is potentially useful for various lab-on-a-chip applications that require the compatibility of system integration without sacrificing mixing efficiency.",

keywords = "High-viscosity mixing, Micromixer, Pulsatile flow, Sputum homogenization",

author = "Korakot Boonyaphon and Zhenglin Li and Geunyong Kim and Lim, {Chae Seung} and Kim, {Sung Jin}",

note = "Funding Information: The study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) ( NRF-2016R1A5A1010148 , 2017R1A2B4005955 ) and was authored as part of Konkuk University{\textquoteright}s research support program for faculty on sabbatical leave in 2018. Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

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AU - Boonyaphon, Korakot

AU - Li, Zhenglin

AU - Kim, Geunyong

AU - Lim, Chae Seung

AU - Kim, Sung Jin

N1 - Funding Information: The study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) ( NRF-2016R1A5A1010148 , 2017R1A2B4005955 ) and was authored as part of Konkuk University’s research support program for faculty on sabbatical leave in 2018. Publisher Copyright: © 2018 Elsevier B.V.

PY - 2018/12/20

Y1 - 2018/12/20

N2 - Homogenizing organism distribution in sputum samples is an initial, yet crucial step to diagnose pulmonary diseases with the samples. Although a homogenization platform for a diagnostic system needs to be system-compatible and efficient, it is rarely implemented. Here, we present a pulsatile micromixer for sputum homogenization that is efficient and does not use dynamic external controllers. The device consists of three oscillator and one mixer units. The oscillator unit autonomously converts a constant input of water-head pressure to a pulsatile pressure, and the mixer unit utilizes this pressure to homogeneously mix two solutions. The mixing performance is analyzed with different oscillation frequencies and width of mixer membranes. The device implemented homogeneous micromixing with a 9 Hz pulse for a flow rate up to 350 μL/min and approximately 200 μL/min for 1 cP and 10 cP viscosity solutions, respectively. Importantly, the device demonstrated successful homogenization of sputum samples with cell viabilities similar to those of the standard method for the flow rate up to 100 μL/min, thereby exhibiting biocompatibility with clinical samples. Our device is potentially useful for various lab-on-a-chip applications that require the compatibility of system integration without sacrificing mixing efficiency.

AB - Homogenizing organism distribution in sputum samples is an initial, yet crucial step to diagnose pulmonary diseases with the samples. Although a homogenization platform for a diagnostic system needs to be system-compatible and efficient, it is rarely implemented. Here, we present a pulsatile micromixer for sputum homogenization that is efficient and does not use dynamic external controllers. The device consists of three oscillator and one mixer units. The oscillator unit autonomously converts a constant input of water-head pressure to a pulsatile pressure, and the mixer unit utilizes this pressure to homogeneously mix two solutions. The mixing performance is analyzed with different oscillation frequencies and width of mixer membranes. The device implemented homogeneous micromixing with a 9 Hz pulse for a flow rate up to 350 μL/min and approximately 200 μL/min for 1 cP and 10 cP viscosity solutions, respectively. Importantly, the device demonstrated successful homogenization of sputum samples with cell viabilities similar to those of the standard method for the flow rate up to 100 μL/min, thereby exhibiting biocompatibility with clinical samples. Our device is potentially useful for various lab-on-a-chip applications that require the compatibility of system integration without sacrificing mixing efficiency.

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Microfluidic sputum homogenizer driven by water-head pressure

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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