Abstract
Background: An increased percentage of pro-inflammatory CD14 +CD16+ monocytes might contribute to inflammation in hemodialysis (HD) patients. The purpose of the study was to evaluate the possible contribution of pro-inflammatory monocytes to inflammation in HD patients and also to evaluate the effect of on-line hemodiafiltration (HDF). Methods: Flow cytometric detection of monocytes in patients undergoing HD, on-line HDF and healthy controls as well as plasma cytokines and cytokine mRNA measurement were performed. Results: Percent pro-inflammatory monocytes, plasma cytokines and cytokine mRNA significantly increased in HD patients. Intracellular cytokine staining showed pro-inflammatory monocytes were the predominant source of tumor necrosis factor-α. Percent pro-inflammatory monocytes positively correlated with plasma inflammatory cytokines. Percent pro-inflammatory monocytes, plasma cytokines and cytokine mRNA significantly decreased in on-line HDF patients. Conclusion: Increased pro-inflammatory monocytes are likely to contribute to inflammation in HD patients, and beneficial effect of on-line HDF might be partially mediated by modulating the inflammatory response.
Original language | English |
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Pages (from-to) | 281-288 |
Number of pages | 8 |
Journal | Blood Purification |
Volume | 31 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2011 Jun |
Externally published | Yes |
Keywords
- Chronic inflammation
- Hemodialysis
- Monocytes
- On-line hemodiafiltration
- Tumor necrosis factor
ASJC Scopus subject areas
- Hematology
- Nephrology