Microinjection free delivery of miRNA inhibitor into zygotes

Jin Young Joo; Jonghwan Lee; Hae Young Ko; Yong Seung Lee; Do Hwan Lim; Eun Young Kim; Sujeong Cho; Ki Sung Hong; Jung Jae Ko; Suman Lee; Young Sik Lee; Youngsok Choi; Kyung Ah Lee; Soonhag Kim

doi:10.1038/srep05417

Microinjection free delivery of miRNA inhibitor into zygotes

Jin Young Joo, Jonghwan Lee, Hae Young Ko, Yong Seung Lee, Do Hwan Lim, Eun Young Kim, Sujeong Cho, Ki Sung Hong, Jung Jae Ko, Suman Lee, Young Sik Lee, Youngsok Choi, Kyung Ah Lee, Soonhag Kim

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

The development of gene delivery systems into embryos is challenging due to technical difficulties, delivery efficiency and toxicity. Here, we developed an organic compound (VisuFect)-mediated gene delivery system for zygotes. The VisuFect, which is hydrophilic and Cy5.5-labeled, was conjugated with poly(A) oligo (VFA). The VFA into CHO cells showed clathrin-mediated internalization and no toxicity. The VFA successfully penetrated through the zona pellucida of fertilized eggs of various species including pigs, zebrafish, drosophilas and mice. The experiment with VisuFect-mediated delivery of the miR34c inhibitor showed similar results with direct microinjection of the miR34c inhibitor by suppressing the development of zygotes up to the blastocyst stage. Noticeable features of the VisuFect will provide great benefits for further studies on gene function in sperms and embryos.

Original language	English
Article number	5417
Journal	Scientific reports
Volume	4
DOIs	https://doi.org/10.1038/srep05417
Publication status	Published - 2014 Jun 24

Bibliographical note

Funding Information:
This work was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MEST) (No. 2012-0006097 and No. 2013R1A2A2A01068140), the Next-Generation BioGreen 21 program (#PJ010002), Rural Development Administration and a grant of the Korea Healthcare technology R&D Project, Ministry of Health and Welfare (A120254).

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title = "Microinjection free delivery of miRNA inhibitor into zygotes",

abstract = "The development of gene delivery systems into embryos is challenging due to technical difficulties, delivery efficiency and toxicity. Here, we developed an organic compound (VisuFect)-mediated gene delivery system for zygotes. The VisuFect, which is hydrophilic and Cy5.5-labeled, was conjugated with poly(A) oligo (VFA). The VFA into CHO cells showed clathrin-mediated internalization and no toxicity. The VFA successfully penetrated through the zona pellucida of fertilized eggs of various species including pigs, zebrafish, drosophilas and mice. The experiment with VisuFect-mediated delivery of the miR34c inhibitor showed similar results with direct microinjection of the miR34c inhibitor by suppressing the development of zygotes up to the blastocyst stage. Noticeable features of the VisuFect will provide great benefits for further studies on gene function in sperms and embryos.",

author = "Joo, {Jin Young} and Jonghwan Lee and Ko, {Hae Young} and Lee, {Yong Seung} and Lim, {Do Hwan} and Kim, {Eun Young} and Sujeong Cho and Hong, {Ki Sung} and Ko, {Jung Jae} and Suman Lee and Lee, {Young Sik} and Youngsok Choi and Lee, {Kyung Ah} and Soonhag Kim",

note = "Funding Information: This work was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MEST) (No. 2012-0006097 and No. 2013R1A2A2A01068140), the Next-Generation BioGreen 21 program (#PJ010002), Rural Development Administration and a grant of the Korea Healthcare technology R&D Project, Ministry of Health and Welfare (A120254).",

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doi = "10.1038/srep05417",

language = "English",

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AU - Joo, Jin Young

AU - Lee, Jonghwan

AU - Ko, Hae Young

AU - Lee, Yong Seung

AU - Lim, Do Hwan

AU - Kim, Eun Young

AU - Cho, Sujeong

AU - Hong, Ki Sung

AU - Ko, Jung Jae

AU - Lee, Suman

AU - Lee, Young Sik

AU - Choi, Youngsok

AU - Lee, Kyung Ah

AU - Kim, Soonhag

N1 - Funding Information: This work was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MEST) (No. 2012-0006097 and No. 2013R1A2A2A01068140), the Next-Generation BioGreen 21 program (#PJ010002), Rural Development Administration and a grant of the Korea Healthcare technology R&D Project, Ministry of Health and Welfare (A120254).

PY - 2014/6/24

Y1 - 2014/6/24

N2 - The development of gene delivery systems into embryos is challenging due to technical difficulties, delivery efficiency and toxicity. Here, we developed an organic compound (VisuFect)-mediated gene delivery system for zygotes. The VisuFect, which is hydrophilic and Cy5.5-labeled, was conjugated with poly(A) oligo (VFA). The VFA into CHO cells showed clathrin-mediated internalization and no toxicity. The VFA successfully penetrated through the zona pellucida of fertilized eggs of various species including pigs, zebrafish, drosophilas and mice. The experiment with VisuFect-mediated delivery of the miR34c inhibitor showed similar results with direct microinjection of the miR34c inhibitor by suppressing the development of zygotes up to the blastocyst stage. Noticeable features of the VisuFect will provide great benefits for further studies on gene function in sperms and embryos.

AB - The development of gene delivery systems into embryos is challenging due to technical difficulties, delivery efficiency and toxicity. Here, we developed an organic compound (VisuFect)-mediated gene delivery system for zygotes. The VisuFect, which is hydrophilic and Cy5.5-labeled, was conjugated with poly(A) oligo (VFA). The VFA into CHO cells showed clathrin-mediated internalization and no toxicity. The VFA successfully penetrated through the zona pellucida of fertilized eggs of various species including pigs, zebrafish, drosophilas and mice. The experiment with VisuFect-mediated delivery of the miR34c inhibitor showed similar results with direct microinjection of the miR34c inhibitor by suppressing the development of zygotes up to the blastocyst stage. Noticeable features of the VisuFect will provide great benefits for further studies on gene function in sperms and embryos.

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Microinjection free delivery of miRNA inhibitor into zygotes

Abstract

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