Micromechanical observation of the kinetics of biomolecular interactions

Taeyun Kwon; Kilho Eom; Jinsung Park; Dae Sung Yoon; Hong Lim Lee; Tae Song Kim

doi:10.1063/1.3006329

Micromechanical observation of the kinetics of biomolecular interactions

Taeyun Kwon, Kilho Eom, Jinsung Park, Dae Sung Yoon, Hong Lim Lee, Tae Song Kim

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

Resonant microcantilevers have recently enabled the label-free detection of biomolecules. Here, we observed the kinetics of biomolecular interactions such as antigen-antibody interactions and/or DNA hybridization based on a resonant frequency shift, obeying Langmuir kinetics, measured in a buffer solution. It is shown that the kinetics of DNA adsorptions on the surface is governed by intermolecular interactions between adsorbed DNA molecules. It is also shown that the kinetics of DNA hybridization is determined by the intermolecular interaction. It is implied that resonant microcantilever in buffer solution may allow for gaining insights into the kinetics of various molecular interactions.

Original language	English
Article number	173901
Journal	Applied Physics Letters
Volume	93
Issue number	17
DOIs	https://doi.org/10.1063/1.3006329
Publication status	Published - 2008
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by Intelligent Microsystem Center sponsored by Korea Ministry of Science and Technology as a part of the 21st Century’s Frontier R&D project (Grant No. MS-01-133-01). First two authors equally contributed to this work.

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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title = "Micromechanical observation of the kinetics of biomolecular interactions",

abstract = "Resonant microcantilevers have recently enabled the label-free detection of biomolecules. Here, we observed the kinetics of biomolecular interactions such as antigen-antibody interactions and/or DNA hybridization based on a resonant frequency shift, obeying Langmuir kinetics, measured in a buffer solution. It is shown that the kinetics of DNA adsorptions on the surface is governed by intermolecular interactions between adsorbed DNA molecules. It is also shown that the kinetics of DNA hybridization is determined by the intermolecular interaction. It is implied that resonant microcantilever in buffer solution may allow for gaining insights into the kinetics of various molecular interactions.",

author = "Taeyun Kwon and Kilho Eom and Jinsung Park and Yoon, {Dae Sung} and Lee, {Hong Lim} and Kim, {Tae Song}",

note = "Funding Information: This work was supported by Intelligent Microsystem Center sponsored by Korea Ministry of Science and Technology as a part of the 21st Century{\textquoteright}s Frontier R&D project (Grant No. MS-01-133-01). First two authors equally contributed to this work. ",

year = "2008",

doi = "10.1063/1.3006329",

language = "English",

volume = "93",

journal = "Applied Physics Letters",

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T1 - Micromechanical observation of the kinetics of biomolecular interactions

AU - Kwon, Taeyun

AU - Eom, Kilho

AU - Park, Jinsung

AU - Yoon, Dae Sung

AU - Lee, Hong Lim

AU - Kim, Tae Song

N1 - Funding Information: This work was supported by Intelligent Microsystem Center sponsored by Korea Ministry of Science and Technology as a part of the 21st Century’s Frontier R&D project (Grant No. MS-01-133-01). First two authors equally contributed to this work.

PY - 2008

Y1 - 2008

N2 - Resonant microcantilevers have recently enabled the label-free detection of biomolecules. Here, we observed the kinetics of biomolecular interactions such as antigen-antibody interactions and/or DNA hybridization based on a resonant frequency shift, obeying Langmuir kinetics, measured in a buffer solution. It is shown that the kinetics of DNA adsorptions on the surface is governed by intermolecular interactions between adsorbed DNA molecules. It is also shown that the kinetics of DNA hybridization is determined by the intermolecular interaction. It is implied that resonant microcantilever in buffer solution may allow for gaining insights into the kinetics of various molecular interactions.

AB - Resonant microcantilevers have recently enabled the label-free detection of biomolecules. Here, we observed the kinetics of biomolecular interactions such as antigen-antibody interactions and/or DNA hybridization based on a resonant frequency shift, obeying Langmuir kinetics, measured in a buffer solution. It is shown that the kinetics of DNA adsorptions on the surface is governed by intermolecular interactions between adsorbed DNA molecules. It is also shown that the kinetics of DNA hybridization is determined by the intermolecular interaction. It is implied that resonant microcantilever in buffer solution may allow for gaining insights into the kinetics of various molecular interactions.

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JO - Applied Physics Letters

JF - Applied Physics Letters

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Micromechanical observation of the kinetics of biomolecular interactions

Abstract

Bibliographical note

ASJC Scopus subject areas

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