Resonant microcantilevers have recently enabled the label-free detection of biomolecules. Here, we observed the kinetics of biomolecular interactions such as antigen-antibody interactions and/or DNA hybridization based on a resonant frequency shift, obeying Langmuir kinetics, measured in a buffer solution. It is shown that the kinetics of DNA adsorptions on the surface is governed by intermolecular interactions between adsorbed DNA molecules. It is also shown that the kinetics of DNA hybridization is determined by the intermolecular interaction. It is implied that resonant microcantilever in buffer solution may allow for gaining insights into the kinetics of various molecular interactions.
Bibliographical noteFunding Information:
This work was supported by Intelligent Microsystem Center sponsored by Korea Ministry of Science and Technology as a part of the 21st Century’s Frontier R&D project (Grant No. MS-01-133-01). First two authors equally contributed to this work.
ASJC Scopus subject areas
- Physics and Astronomy (miscellaneous)