The inability of neurons to undergo mitosis renders damage to the central or peripheral nervous system. Neural stem cell therapy could provide a path for treating the neurodegenerative diseases. However, reliable and simple tools for the developing and testing neural stem cell therapy are still required. Here, we show the development of a micropillar-based microfluidic device to trap the uniform-sized neurospheres. The neurospheres trapped within micropillar arrays were largely differentiated into neuronal cells, and their neurite networks were observed in the microfluidic device. Compared to conventional cultures on glass slides, the neurite networks generated with this method have a higher reproducibility. Furthermore, we demonstrated the effect of thapsigargin on the neurite networks in the microfluidic device, demonstrating that neural networks exposed to thapsigargin were largely diminished and disconnected from each other. Therefore, this micropillar-based microfluidic device could be a potential tool for screening of neurotoxins.
Bibliographical noteFunding Information:
This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute, funded by the Ministry of Health & Welfare, Republic of Korea, (Grant number HI14C3347). This work was supported by the National Research Foundation (NRF) of Korea grant funded by the Ministry of Science and ICT (MSIT) (Grant number 2017R1C1B3012221). This work was also supported by Leading Foreign Research Institute Recruitment Program through the NRF of Korea funded by the MSIT (Grant number 2013K1A4A3055268).
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
- Micropillar array
- Neurite network
ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry