Migrasomal autophagosomes relieve endoplasmic reticulum stress in glioblastoma cells

Seon Yong Lee, Sang Hun Choi, Yoonji Kim, Hee Sung Ahn, Young Gyu Ko, Kyunggon Kim, Sung Wook Chi, Hyunggee Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Glioblastoma (GBM) is more difficult to treat than other intractable adult tumors. The main reason that GBM is so difficult to treat is that it is highly infiltrative. Migrasomes are newly discovered membrane structures observed in migrating cells. Thus, they can be generated from GBM cells that have the ability to migrate along the brain parenchyma. However, the function of migrasomes has not yet been elucidated in GBM cells. Results: Here, we describe the composition and function of migrasomes generated along with GBM cell migration. Proteomic analysis revealed that LC3B-positive autophagosomes were abundant in the migrasomes of GBM cells. An increased number of migrasomes was observed following treatment with chloroquine (CQ) or inhibition of the expression of STX17 and SNAP29, which are involved in autophagosome/lysosome fusion. Furthermore, depletion of ITGA5 or TSPAN4 did not relieve endoplasmic reticulum (ER) stress in cells, resulting in cell death. Conclusions: Taken together, our study suggests that increasing the number of autophagosomes, through inhibition of autophagosome/lysosome fusion, generates migrasomes that have the capacity to alleviate cellular stress.

Original languageEnglish
Article number23
JournalBMC biology
Volume22
Issue number1
DOIs
Publication statusPublished - 2024 Dec

Bibliographical note

Publisher Copyright:
© 2024, The Author(s).

Keywords

  • Autophagosome
  • Cell death
  • ER stress
  • ITGA5
  • Migrasome
  • Retraction fiber
  • TSPAN4

ASJC Scopus subject areas

  • Biotechnology
  • Structural Biology
  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • General Biochemistry,Genetics and Molecular Biology
  • General Agricultural and Biological Sciences
  • Plant Science
  • Developmental Biology
  • Cell Biology

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