Abstract
Nano-sized exosome (30-150 nm) is emerging biomarker for liquid biopsy of cancer. However, the conventional method to isolate exosome: ultracentrifugation has various disadvantages such as time consuming (> 6 hr), high cost, etc. Here, we present a novel exosome isolation method using cationic polymer in a miniaturized system including a syringe filter. The present system can isolate exosome in 20 min with 20% higher vesicle yield and comparable exosomal miRNA levels compared to ultracentrifugation.
| Original language | English |
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| Title of host publication | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1600-1601 |
| Number of pages | 2 |
| ISBN (Electronic) | 9781733419000 |
| Publication status | Published - 2019 |
| Event | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland Duration: 2019 Oct 27 → 2019 Oct 31 |
Publication series
| Name | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 |
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Conference
| Conference | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 |
|---|---|
| Country/Territory | Switzerland |
| City | Basel |
| Period | 19/10/27 → 19/10/31 |
Bibliographical note
Funding Information:This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIP) (NRF-2016R1A5A1010148, NRF-2015M3A9D7031026, NRF-2016M3A7B4909649).
Publisher Copyright:
© 2019 CBMS-0001.
Keywords
- Exosome
- Extracellular vesicle
- Isolation
- Polymer
ASJC Scopus subject areas
- Bioengineering
- Chemical Engineering (miscellaneous)
