Miniaturized exosome isolation system using cationic polymer and syringe filter

Chanhee Park, Jinhyun Kim, Hoyoon Lee, Jina Choi, Hyunsung Kim, Sehyun Shin

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Nano-sized exosome (30-150 nm) is emerging biomarker for liquid biopsy of cancer. However, the conventional method to isolate exosome: ultracentrifugation has various disadvantages such as time consuming (> 6 hr), high cost, etc. Here, we present a novel exosome isolation method using cationic polymer in a miniaturized system including a syringe filter. The present system can isolate exosome in 20 min with 20% higher vesicle yield and comparable exosomal miRNA levels compared to ultracentrifugation.

    Original languageEnglish
    Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
    PublisherChemical and Biological Microsystems Society
    Pages1600-1601
    Number of pages2
    ISBN (Electronic)9781733419000
    Publication statusPublished - 2019
    Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
    Duration: 2019 Oct 272019 Oct 31

    Publication series

    Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

    Conference

    Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
    Country/TerritorySwitzerland
    CityBasel
    Period19/10/2719/10/31

    Bibliographical note

    Funding Information:
    This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIP) (NRF-2016R1A5A1010148, NRF-2015M3A9D7031026, NRF-2016M3A7B4909649).

    Publisher Copyright:
    © 2019 CBMS-0001.

    Keywords

    • Exosome
    • Extracellular vesicle
    • Isolation
    • Polymer

    ASJC Scopus subject areas

    • Bioengineering
    • Chemical Engineering (miscellaneous)

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