TY - GEN
T1 - Miniaturized exosome isolation system using cationic polymer and syringe filter
AU - Park, Chanhee
AU - Kim, Jinhyun
AU - Lee, Hoyoon
AU - Choi, Jina
AU - Kim, Hyunsung
AU - Shin, Sehyun
N1 - Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIP) (NRF-2016R1A5A1010148, NRF-2015M3A9D7031026, NRF-2016M3A7B4909649).
Publisher Copyright:
© 2019 CBMS-0001.
PY - 2019
Y1 - 2019
N2 - Nano-sized exosome (30-150 nm) is emerging biomarker for liquid biopsy of cancer. However, the conventional method to isolate exosome: ultracentrifugation has various disadvantages such as time consuming (> 6 hr), high cost, etc. Here, we present a novel exosome isolation method using cationic polymer in a miniaturized system including a syringe filter. The present system can isolate exosome in 20 min with 20% higher vesicle yield and comparable exosomal miRNA levels compared to ultracentrifugation.
AB - Nano-sized exosome (30-150 nm) is emerging biomarker for liquid biopsy of cancer. However, the conventional method to isolate exosome: ultracentrifugation has various disadvantages such as time consuming (> 6 hr), high cost, etc. Here, we present a novel exosome isolation method using cationic polymer in a miniaturized system including a syringe filter. The present system can isolate exosome in 20 min with 20% higher vesicle yield and comparable exosomal miRNA levels compared to ultracentrifugation.
KW - Exosome
KW - Extracellular vesicle
KW - Isolation
KW - Polymer
UR - http://www.scopus.com/inward/record.url?scp=85094950441&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85094950441
T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
SP - 1600
EP - 1601
BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PB - Chemical and Biological Microsystems Society
T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Y2 - 27 October 2019 through 31 October 2019
ER -
