Minicell-forming Escherichia coli mutant with increased chemical production capacity and tolerance to toxic compounds

Seung Jin Kim, Min Kyu Oh

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Minicell, a small spherical form of bacterium produced by abnormal fission, possesses cytoplasmic constituents similar to those of the parental cell, except for genomic DNA. E. coli strains were engineered to produce minicells and value-added chemicals. Minicell-forming mutants showed enhanced tolerance to toxic chemicals and a higher intracellular NADH/NAD+ ratio than the wild-type. When toxic chemicals such as isobutanol, isobutyraldehyde, and isobutyl acetate were produced in this mutant, the titers increased by 67 %, 175 %, and 214 %, respectively. In addition, morphological changes and membrane dispersion mechanisms in minicell-forming mutants improved lycopene production by 259 %. This increase in production capacity was more pronounced when biomass hydrolysate was used as the substrate. Isobutanol and lycopene production also increased by 92 % and 295 %, respectively, on using the substrate in the mutant. It suggests that minicell-forming mutants are an excellent platform for biochemical production.

Original languageEnglish
Article number128586
JournalBioresource technology
Publication statusPublished - 2023 Mar

Bibliographical note

Funding Information:
This work was supported by the C1 Gas Refinery Program through the National Research Foundation of Korea , funded by the Ministry of Science and ICT ( 2015M3D3A1A01064919 ).

Publisher Copyright:
© 2023 Elsevier Ltd


  • Hydrolysate
  • Isobutanol
  • Lycopene
  • Minicell-forming mutant
  • Tolerance

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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