Abstract
The phosphatase of regenerating liver-3 (. PRL-. 3) gene is associated with metastasis in gastric cancer, and is believed to play a causative role by promoting tumor cell motility, invasion, and metastasis, but little is known of the mechanisms involved. We previously reported that . PRL-. 3 expression is significantly higher in the tissues of primary gastric carcinomas with peritoneal metastasis. In the present study, we found that two microRNAs (miRNAs), miR-495 and miR-551a, predicted to target . PRL-. 3, are downregulated in gastric carcinoma samples. The validation of this interaction between those two miRNAs and . PRL-. 3 was confirmed by western blotting and quantitative real-time PCR (qPCR) in GC cell lines transfected with miR-495 and miR-551a mimics. Furthermore, the migration and invasion of GC cells were significantly inhibited by transfection with miR-495 or -551a mimics, and the mRNA and protein levels of . PRL-. 3 were reduced in cells overexpressing miR-495 or -551a. Collectively, our findings suggest that miR-495 and miR-551a both act as tumor suppressors by targeting the . PRL-. 3 oncogene and inhibiting gastric cancer cell migration and invasion. The findings of this study contribute to current understanding of the functions of miRNA mimics in GC gene therapy.
Original language | English |
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Pages (from-to) | 41-47 |
Number of pages | 7 |
Journal | Cancer letters |
Volume | 323 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2012 Oct 1 |
Keywords
- Gastric carcinoma
- MiR-495
- MiR-551a
- PRL-3
ASJC Scopus subject areas
- Oncology
- Cancer Research