MiR-495 and miR-551a inhibit the migration and invasion of human gastric cancer cells by directly interacting with PRL-3

Zhengrong Li; Yi Cao; Zhigang Jie; Yi Liu; Yingliang Li; Junhe Li; Guoming Zhu; Zhengren Liu; Yi Tu; Gen Peng; Dong woo Lee; Sung soo Park

doi:10.1016/j.canlet.2012.03.029

MiR-495 and miR-551a inhibit the migration and invasion of human gastric cancer cells by directly interacting with PRL-3

Zhengrong Li, Yi Cao, Zhigang Jie, Yi Liu, Yingliang Li, Junhe Li, Guoming Zhu, Zhengren Liu, Yi Tu, Gen Peng, Dong woo Lee, Sung soo Park

Research output: Contribution to journal › Article › peer-review

99 Citations (Scopus)

Abstract

The phosphatase of regenerating liver-3 (. PRL-. 3) gene is associated with metastasis in gastric cancer, and is believed to play a causative role by promoting tumor cell motility, invasion, and metastasis, but little is known of the mechanisms involved. We previously reported that . PRL-. 3 expression is significantly higher in the tissues of primary gastric carcinomas with peritoneal metastasis. In the present study, we found that two microRNAs (miRNAs), miR-495 and miR-551a, predicted to target . PRL-. 3, are downregulated in gastric carcinoma samples. The validation of this interaction between those two miRNAs and . PRL-. 3 was confirmed by western blotting and quantitative real-time PCR (qPCR) in GC cell lines transfected with miR-495 and miR-551a mimics. Furthermore, the migration and invasion of GC cells were significantly inhibited by transfection with miR-495 or -551a mimics, and the mRNA and protein levels of . PRL-. 3 were reduced in cells overexpressing miR-495 or -551a. Collectively, our findings suggest that miR-495 and miR-551a both act as tumor suppressors by targeting the . PRL-. 3 oncogene and inhibiting gastric cancer cell migration and invasion. The findings of this study contribute to current understanding of the functions of miRNA mimics in GC gene therapy.

Original language	English
Pages (from-to)	41-47
Number of pages	7
Journal	Cancer letters
Volume	323
Issue number	1
DOIs	https://doi.org/10.1016/j.canlet.2012.03.029
Publication status	Published - 2012 Oct 1

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (NSFC) (Grant Nos. 30901429 and 81160304 ). The authors thank Heping Chen and Zhigang Wang for technical support.

Keywords

Gastric carcinoma
MiR-495
MiR-551a
PRL-3

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.canlet.2012.03.029

Cite this

@article{a5b58e475ddb474d83862f74fe6d7094,

title = "MiR-495 and miR-551a inhibit the migration and invasion of human gastric cancer cells by directly interacting with PRL-3",

abstract = "The phosphatase of regenerating liver-3 (. PRL-. 3) gene is associated with metastasis in gastric cancer, and is believed to play a causative role by promoting tumor cell motility, invasion, and metastasis, but little is known of the mechanisms involved. We previously reported that . PRL-. 3 expression is significantly higher in the tissues of primary gastric carcinomas with peritoneal metastasis. In the present study, we found that two microRNAs (miRNAs), miR-495 and miR-551a, predicted to target . PRL-. 3, are downregulated in gastric carcinoma samples. The validation of this interaction between those two miRNAs and . PRL-. 3 was confirmed by western blotting and quantitative real-time PCR (qPCR) in GC cell lines transfected with miR-495 and miR-551a mimics. Furthermore, the migration and invasion of GC cells were significantly inhibited by transfection with miR-495 or -551a mimics, and the mRNA and protein levels of . PRL-. 3 were reduced in cells overexpressing miR-495 or -551a. Collectively, our findings suggest that miR-495 and miR-551a both act as tumor suppressors by targeting the . PRL-. 3 oncogene and inhibiting gastric cancer cell migration and invasion. The findings of this study contribute to current understanding of the functions of miRNA mimics in GC gene therapy.",

keywords = "Gastric carcinoma, MiR-495, MiR-551a, PRL-3",

author = "Zhengrong Li and Yi Cao and Zhigang Jie and Yi Liu and Yingliang Li and Junhe Li and Guoming Zhu and Zhengren Liu and Yi Tu and Gen Peng and Lee, {Dong woo} and Park, {Sung soo}",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (NSFC) (Grant Nos. 30901429 and 81160304 ). The authors thank Heping Chen and Zhigang Wang for technical support. ",

year = "2012",

month = oct,

day = "1",

doi = "10.1016/j.canlet.2012.03.029",

language = "English",

volume = "323",

pages = "41--47",

journal = "Cancer letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - MiR-495 and miR-551a inhibit the migration and invasion of human gastric cancer cells by directly interacting with PRL-3

AU - Li, Zhengrong

AU - Cao, Yi

AU - Jie, Zhigang

AU - Liu, Yi

AU - Li, Yingliang

AU - Li, Junhe

AU - Zhu, Guoming

AU - Liu, Zhengren

AU - Tu, Yi

AU - Peng, Gen

AU - Lee, Dong woo

AU - Park, Sung soo

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (NSFC) (Grant Nos. 30901429 and 81160304 ). The authors thank Heping Chen and Zhigang Wang for technical support.

PY - 2012/10/1

Y1 - 2012/10/1

N2 - The phosphatase of regenerating liver-3 (. PRL-. 3) gene is associated with metastasis in gastric cancer, and is believed to play a causative role by promoting tumor cell motility, invasion, and metastasis, but little is known of the mechanisms involved. We previously reported that . PRL-. 3 expression is significantly higher in the tissues of primary gastric carcinomas with peritoneal metastasis. In the present study, we found that two microRNAs (miRNAs), miR-495 and miR-551a, predicted to target . PRL-. 3, are downregulated in gastric carcinoma samples. The validation of this interaction between those two miRNAs and . PRL-. 3 was confirmed by western blotting and quantitative real-time PCR (qPCR) in GC cell lines transfected with miR-495 and miR-551a mimics. Furthermore, the migration and invasion of GC cells were significantly inhibited by transfection with miR-495 or -551a mimics, and the mRNA and protein levels of . PRL-. 3 were reduced in cells overexpressing miR-495 or -551a. Collectively, our findings suggest that miR-495 and miR-551a both act as tumor suppressors by targeting the . PRL-. 3 oncogene and inhibiting gastric cancer cell migration and invasion. The findings of this study contribute to current understanding of the functions of miRNA mimics in GC gene therapy.

AB - The phosphatase of regenerating liver-3 (. PRL-. 3) gene is associated with metastasis in gastric cancer, and is believed to play a causative role by promoting tumor cell motility, invasion, and metastasis, but little is known of the mechanisms involved. We previously reported that . PRL-. 3 expression is significantly higher in the tissues of primary gastric carcinomas with peritoneal metastasis. In the present study, we found that two microRNAs (miRNAs), miR-495 and miR-551a, predicted to target . PRL-. 3, are downregulated in gastric carcinoma samples. The validation of this interaction between those two miRNAs and . PRL-. 3 was confirmed by western blotting and quantitative real-time PCR (qPCR) in GC cell lines transfected with miR-495 and miR-551a mimics. Furthermore, the migration and invasion of GC cells were significantly inhibited by transfection with miR-495 or -551a mimics, and the mRNA and protein levels of . PRL-. 3 were reduced in cells overexpressing miR-495 or -551a. Collectively, our findings suggest that miR-495 and miR-551a both act as tumor suppressors by targeting the . PRL-. 3 oncogene and inhibiting gastric cancer cell migration and invasion. The findings of this study contribute to current understanding of the functions of miRNA mimics in GC gene therapy.

KW - Gastric carcinoma

KW - MiR-495

KW - MiR-551a

KW - PRL-3

UR - http://www.scopus.com/inward/record.url?scp=84861807243&partnerID=8YFLogxK

U2 - 10.1016/j.canlet.2012.03.029

DO - 10.1016/j.canlet.2012.03.029

M3 - Article

C2 - 22469786

AN - SCOPUS:84861807243

SN - 0304-3835

VL - 323

SP - 41

EP - 47

JO - Cancer letters

JF - Cancer letters

IS - 1

ER -

MiR-495 and miR-551a inhibit the migration and invasion of human gastric cancer cells by directly interacting with PRL-3

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this