Mitochondria-immobilized ph-sensitive off-on fluorescent probe

Min Hee Lee; Nayoung Park; Chunsik Yi; Ji Hye Han; Ji Hye Hong; Kwang Pyo Kim; Dong Hoon Kang; Jonathan L. Sessler; Chulhun Kang; Jong Seung Kim

doi:10.1021/ja506301n

Mitochondria-immobilized ph-sensitive off-on fluorescent probe

Min Hee Lee, Nayoung Park, Chunsik Yi, Ji Hye Han, Ji Hye Hong, Kwang Pyo Kim, Dong Hoon Kang, Jonathan L. Sessler, Chulhun Kang, Jong Seung Kim

Research output: Contribution to journal › Article › peer-review

360 Citations (Scopus)

Abstract

We report here a mitochondria-targetable pH-sensitive probe that allows for a quantitative measurement of mitochondrial pH changes, as well as the real-time monitoring of pH-related physiological effects in live cells. This system consists of a piperazine-linked naphthalimide as a fluorescence off-on signaling unit, a cationic triphenylphosphonium group for mitochondrial targeting, and a reactive benzyl chloride subunit for mitochondrial fixation. It operates well in a mitochondrial environment within whole cells and displays a desirable off-on fluorescence response to mitochondrial acidification. Moreover, this probe allows for the monitoring of impaired mitochondria undergoing mitophagic elimination as the result of nutrient starvation. It thus allows for the monitoring of the organelle-specific dynamics associated with the conversion between physiological and pathological states.

Original language	English
Pages (from-to)	14136-14142
Number of pages	7
Journal	Journal of the American Chemical Society
Volume	136
Issue number	40
DOIs	https://doi.org/10.1021/ja506301n
Publication status	Published - 2014 Oct 8

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

Access to Document

10.1021/ja506301n

Cite this

@article{64a0d188ce8f49f0b6e2cb865fac2cd1,

title = "Mitochondria-immobilized ph-sensitive off-on fluorescent probe",

abstract = "We report here a mitochondria-targetable pH-sensitive probe that allows for a quantitative measurement of mitochondrial pH changes, as well as the real-time monitoring of pH-related physiological effects in live cells. This system consists of a piperazine-linked naphthalimide as a fluorescence off-on signaling unit, a cationic triphenylphosphonium group for mitochondrial targeting, and a reactive benzyl chloride subunit for mitochondrial fixation. It operates well in a mitochondrial environment within whole cells and displays a desirable off-on fluorescence response to mitochondrial acidification. Moreover, this probe allows for the monitoring of impaired mitochondria undergoing mitophagic elimination as the result of nutrient starvation. It thus allows for the monitoring of the organelle-specific dynamics associated with the conversion between physiological and pathological states.",

author = "Lee, {Min Hee} and Nayoung Park and Chunsik Yi and Han, {Ji Hye} and Hong, {Ji Hye} and Kim, {Kwang Pyo} and Kang, {Dong Hoon} and Sessler, {Jonathan L.} and Chulhun Kang and Kim, {Jong Seung}",

note = "Publisher Copyright: {\textcopyright} 2014 American Chemical Society.",

year = "2014",

month = oct,

day = "8",

doi = "10.1021/ja506301n",

language = "English",

volume = "136",

pages = "14136--14142",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "40",

}

TY - JOUR

T1 - Mitochondria-immobilized ph-sensitive off-on fluorescent probe

AU - Lee, Min Hee

AU - Park, Nayoung

AU - Yi, Chunsik

AU - Han, Ji Hye

AU - Hong, Ji Hye

AU - Kim, Kwang Pyo

AU - Kang, Dong Hoon

AU - Sessler, Jonathan L.

AU - Kang, Chulhun

AU - Kim, Jong Seung

PY - 2014/10/8

Y1 - 2014/10/8

N2 - We report here a mitochondria-targetable pH-sensitive probe that allows for a quantitative measurement of mitochondrial pH changes, as well as the real-time monitoring of pH-related physiological effects in live cells. This system consists of a piperazine-linked naphthalimide as a fluorescence off-on signaling unit, a cationic triphenylphosphonium group for mitochondrial targeting, and a reactive benzyl chloride subunit for mitochondrial fixation. It operates well in a mitochondrial environment within whole cells and displays a desirable off-on fluorescence response to mitochondrial acidification. Moreover, this probe allows for the monitoring of impaired mitochondria undergoing mitophagic elimination as the result of nutrient starvation. It thus allows for the monitoring of the organelle-specific dynamics associated with the conversion between physiological and pathological states.

AB - We report here a mitochondria-targetable pH-sensitive probe that allows for a quantitative measurement of mitochondrial pH changes, as well as the real-time monitoring of pH-related physiological effects in live cells. This system consists of a piperazine-linked naphthalimide as a fluorescence off-on signaling unit, a cationic triphenylphosphonium group for mitochondrial targeting, and a reactive benzyl chloride subunit for mitochondrial fixation. It operates well in a mitochondrial environment within whole cells and displays a desirable off-on fluorescence response to mitochondrial acidification. Moreover, this probe allows for the monitoring of impaired mitochondria undergoing mitophagic elimination as the result of nutrient starvation. It thus allows for the monitoring of the organelle-specific dynamics associated with the conversion between physiological and pathological states.

UR - http://www.scopus.com/inward/record.url?scp=84907942519&partnerID=8YFLogxK

U2 - 10.1021/ja506301n

DO - 10.1021/ja506301n

M3 - Article

C2 - 25158001

AN - SCOPUS:84907942519

SN - 0002-7863

VL - 136

SP - 14136

EP - 14142

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 40

ER -

Mitochondria-immobilized ph-sensitive off-on fluorescent probe

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this