Mitochondrial oxidative phosphorylation complexes exist in the sarcolemma of skeletal muscle

Hyun Lee, Seung Hyeob Kim, Jae Seon Lee, Yun Hee Yang, Jwa Min Nam, Bong Woo Kim, Young Gyu Ko

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    Although proteomic analyses have revealed the presence of mitochondrial oxidative phosphorylation (OXPHOS) proteins in the plasma membrane, there have been no in-depth evaluations of the presence or function of OXPHOS I-V in the plasma membrane. Here, we demonstrate the in situ localization of OXPHOS I-V complexes to the sarcolemma of skeletal muscle by immunofluorescence and immunohistochemistry. A portion of the OXPHOS I-V complex proteins was not co-stained with MitoTracker but co-localized with caveolin-3 in the sarcolemma of mouse gastrocnemius. Mitochondrial matrix-facing OXPHOS complex subunits were ectopically expressed in the sarcolemma of the non-permeabilized muscle fibers and C2C12 myotubes. The sarcolemmal localization of cytochrome c was also observed from mouse gastrocnemius muscles and C2C12 myotubes, as determined by confocal and total internal resonance fluorescence (TIRF) microscopy. Based on these data, we conclude that a portion of OXPHOS complexes is localized in the sarcolemma of skeletal muscle and may have non-canonical functions.

    Original languageEnglish
    Pages (from-to)116-121
    Number of pages6
    JournalBMB reports
    Volume49
    Issue number2
    DOIs
    Publication statusPublished - 2016

    Bibliographical note

    Funding Information:
    This work was supported by a grant awarded to Y.-G. Ko from the National Research Foundation (2011-0017562).

    Keywords

    • Cytochrome c
    • Extracellular NADH-dependent respiration
    • Oxidative phosphorylation complexes
    • Sarcolemma
    • Skeletal muscle

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

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