Modeling of three-dimensional innervated epidermal like-layer in a microfluidic chip-based coculture system

Jinchul Ahn; Kyungeun Ohk; Jihee Won; Dong Hee Choi; Yong Hun Jung; Ji Hun Yang; Yesl Jun; Jin A. Kim; Seok Chung; Sang Hoon Lee

doi:10.1038/s41467-023-37187-4

Modeling of three-dimensional innervated epidermal like-layer in a microfluidic chip-based coculture system

Jinchul Ahn, Kyungeun Ohk, Jihee Won, Dong Hee Choi, Yong Hun Jung, Ji Hun Yang, Yesl Jun, Jin A. Kim, Seok Chung, Sang Hoon Lee

School of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Reconstruction of skin equivalents with physiologically relevant cellular and matrix architecture is indispensable for basic research and industrial applications. As skin-nerve crosstalk is increasingly recognized as a major element of skin physiological pathology, the development of reliable in vitro models to evaluate the selective communication between epidermal keratinocytes and sensory neurons is being demanded. In this study, we present a three-dimensional innervated epidermal keratinocyte layer as a sensory neuron-epidermal keratinocyte co-culture model on a microfluidic chip using the slope-based air-liquid interfacing culture and spatial compartmentalization. Our co-culture model recapitulates a more organized basal-suprabasal stratification, enhanced barrier function, and physiologically relevant anatomical innervation and demonstrated the feasibility of in situ imaging and functional analysis in a cell-type-specific manner, thereby improving the structural and functional limitations of previous coculture models. This system has the potential as an improved surrogate model and platform for biomedical and pharmaceutical research.

Original language	English
Article number	1488
Journal	Nature communications
Volume	14
Issue number	1
DOIs	https://doi.org/10.1038/s41467-023-37187-4
Publication status	Published - 2023 Dec

Bibliographical note

Funding Information:
This work was supported by Samsung Research Funding & Incubation Center of Samsung Electronics under Project Number SRFC-IT1901-51 and by the Korea Evaluation Institute of Industrial Technology (KEIT) grant funded by the Korea government (MSIT) (No. 20009125). JA Kim was supported by the Technology Innovation Program (or Industrial Strategic Technology Development Program (20015148, Development of Neural/Vascular/Muscular-Specific Peptides-conjugated Bioink and Volumetric Muscle Tissue) funded By the Ministry of Trade, Industry & Energy (MOTIE, Korea).

Publisher Copyright:
© 2023, The Author(s).

ASJC Scopus subject areas

Physics and Astronomy(all)
Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)

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10.1038/s41467-023-37187-4

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title = "Modeling of three-dimensional innervated epidermal like-layer in a microfluidic chip-based coculture system",

abstract = "Reconstruction of skin equivalents with physiologically relevant cellular and matrix architecture is indispensable for basic research and industrial applications. As skin-nerve crosstalk is increasingly recognized as a major element of skin physiological pathology, the development of reliable in vitro models to evaluate the selective communication between epidermal keratinocytes and sensory neurons is being demanded. In this study, we present a three-dimensional innervated epidermal keratinocyte layer as a sensory neuron-epidermal keratinocyte co-culture model on a microfluidic chip using the slope-based air-liquid interfacing culture and spatial compartmentalization. Our co-culture model recapitulates a more organized basal-suprabasal stratification, enhanced barrier function, and physiologically relevant anatomical innervation and demonstrated the feasibility of in situ imaging and functional analysis in a cell-type-specific manner, thereby improving the structural and functional limitations of previous coculture models. This system has the potential as an improved surrogate model and platform for biomedical and pharmaceutical research.",

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AU - Yang, Ji Hun

AU - Jun, Yesl

AU - Kim, Jin A.

AU - Chung, Seok

AU - Lee, Sang Hoon

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Modeling of three-dimensional innervated epidermal like-layer in a microfluidic chip-based coculture system

Abstract

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