Abstract
Modified floating electrode-based sensors were developed to quantitatively monitor the levels of tumor necrosis factor α (TNF-α), a pro-inflammatory cytokine related with inflammatory bowel disease (IBD), and to evaluate the effect of drugs on the cytokine levels. Here, antibodies (anti-TNF-α) were immobilized on the floating electrodes of carbon nanotube devices, enabling selective and real-time detection of TNF-α among various cytokines linked to IBD. This sensor was able to measure the concentrations of TNF-α with a detection limit of 1 pg/L, allowing the quantitative estimation of TNF-α secretion from mouse macrophage Raw 264.7 cells stimulated by lipopolysaccharides (LPS). Notably, this method also allowed us to monitor the anti-inflammatory effect of a drug, lupeol, on the activation of the LPS-induced nuclear factor κB signaling in Raw 264.7 cells. These results indicate that our novel TNF sensor can be a versatile tool for biomedical research and clinical applications such as screening drug effects and monitoring inflammation levels.
Original language | English |
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Pages (from-to) | 17100-17106 |
Number of pages | 7 |
Journal | ACS Applied Materials and Interfaces |
Volume | 10 |
Issue number | 20 |
DOIs | |
Publication status | Published - 2018 May 23 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:Copyright © 2018 American Chemical Society.
Keywords
- Raw 264.7 cells
- carbon nanotube field-effect transistors
- lipopolysaccharides
- lupeol
- tumor necrosis factor α
ASJC Scopus subject areas
- General Materials Science