Modulating the GSH/Trx selectivity of a fluorogenic disulfide-based thiol sensor to reveal diminished GSH levels under ER stress

Youngjin Wi; Hoa Thi Le; Peter Verwilst; Kyoung Sunwoo; Seo Jin Kim; Jung Eun Song; Hey Young Yoon; Geon Han; Jong Seung Kim; Chulhun Kang; Tae Woo Kim

doi:10.1039/c8cc04846k

Modulating the GSH/Trx selectivity of a fluorogenic disulfide-based thiol sensor to reveal diminished GSH levels under ER stress

Youngjin Wi, Hoa Thi Le, Peter Verwilst, Kyoung Sunwoo, Seo Jin Kim, Jung Eun Song, Hey Young Yoon, Geon Han, Jong Seung Kim, Chulhun Kang, Tae Woo Kim

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

We synthesized a fluorogenic disulfide-based naphthalimide thiol probe (ER-Naph) with a hydrophilic endoplasmic reticulum (ER)-guiding glibenclamide unit. Its ER targeting ability and high selectivity to GSH over thioredoxin, a potent competitor, were clearly demonstrated, both in solution and in vitro. Finally, a confocal microscopic investigation revealed that GSH levels in the ER were dramatically decreased under thapsigargin, brefeldin A, and tunicamycin-induced ER stress models.

Original language	English
Pages (from-to)	8897-8900
Number of pages	4
Journal	Chemical Communications
Volume	54
Issue number	64
DOIs	https://doi.org/10.1039/c8cc04846k
Publication status	Published - 2018

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

Access to Document

10.1039/c8cc04846k

Cite this

@article{109e40352b96478ba5690938473014c7,

title = "Modulating the GSH/Trx selectivity of a fluorogenic disulfide-based thiol sensor to reveal diminished GSH levels under ER stress",

abstract = "We synthesized a fluorogenic disulfide-based naphthalimide thiol probe (ER-Naph) with a hydrophilic endoplasmic reticulum (ER)-guiding glibenclamide unit. Its ER targeting ability and high selectivity to GSH over thioredoxin, a potent competitor, were clearly demonstrated, both in solution and in vitro. Finally, a confocal microscopic investigation revealed that GSH levels in the ER were dramatically decreased under thapsigargin, brefeldin A, and tunicamycin-induced ER stress models.",

author = "Youngjin Wi and Le, {Hoa Thi} and Peter Verwilst and Kyoung Sunwoo and Kim, {Seo Jin} and Song, {Jung Eun} and Yoon, {Hey Young} and Geon Han and Kim, {Jong Seung} and Chulhun Kang and Kim, {Tae Woo}",

note = "Funding Information: This work was supported by CRI (No. 2009-0081566, JSK), NRF (No. 2017R1A2A2A05069805, CK), and NRF (No. 2017 R1D1A1B03034972, TWK) grants funded by the Ministry of Science and ICT through the National Research Foundation of Korea. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",

year = "2018",

doi = "10.1039/c8cc04846k",

language = "English",

volume = "54",

pages = "8897--8900",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "64",

}

TY - JOUR

T1 - Modulating the GSH/Trx selectivity of a fluorogenic disulfide-based thiol sensor to reveal diminished GSH levels under ER stress

AU - Wi, Youngjin

AU - Le, Hoa Thi

AU - Verwilst, Peter

AU - Sunwoo, Kyoung

AU - Kim, Seo Jin

AU - Song, Jung Eun

AU - Yoon, Hey Young

AU - Han, Geon

AU - Kim, Jong Seung

AU - Kang, Chulhun

AU - Kim, Tae Woo

N1 - Funding Information: This work was supported by CRI (No. 2009-0081566, JSK), NRF (No. 2017R1A2A2A05069805, CK), and NRF (No. 2017 R1D1A1B03034972, TWK) grants funded by the Ministry of Science and ICT through the National Research Foundation of Korea. Publisher Copyright: © The Royal Society of Chemistry.

PY - 2018

Y1 - 2018

N2 - We synthesized a fluorogenic disulfide-based naphthalimide thiol probe (ER-Naph) with a hydrophilic endoplasmic reticulum (ER)-guiding glibenclamide unit. Its ER targeting ability and high selectivity to GSH over thioredoxin, a potent competitor, were clearly demonstrated, both in solution and in vitro. Finally, a confocal microscopic investigation revealed that GSH levels in the ER were dramatically decreased under thapsigargin, brefeldin A, and tunicamycin-induced ER stress models.

AB - We synthesized a fluorogenic disulfide-based naphthalimide thiol probe (ER-Naph) with a hydrophilic endoplasmic reticulum (ER)-guiding glibenclamide unit. Its ER targeting ability and high selectivity to GSH over thioredoxin, a potent competitor, were clearly demonstrated, both in solution and in vitro. Finally, a confocal microscopic investigation revealed that GSH levels in the ER were dramatically decreased under thapsigargin, brefeldin A, and tunicamycin-induced ER stress models.

UR - http://www.scopus.com/inward/record.url?scp=85051273116&partnerID=8YFLogxK

U2 - 10.1039/c8cc04846k

DO - 10.1039/c8cc04846k

M3 - Article

C2 - 30043021

AN - SCOPUS:85051273116

SN - 1359-7345

VL - 54

SP - 8897

EP - 8900

JO - Chemical Communications

JF - Chemical Communications

IS - 64

ER -

Modulating the GSH/Trx selectivity of a fluorogenic disulfide-based thiol sensor to reveal diminished GSH levels under ER stress

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this