Modulation of Macrophages by In Situ Ligand Bridging

Seong Yeol Kim, Ramar Thangam, Nayeon Kang, Hyunsik Hong, Chowon Kim, Sungkyu Lee, Subin Son, Hyun Jeong Lee, Kyong Ryol Tag, Sunhong Min, Daun Jeong, Jangsun Hwang, Kanghyeon Kim, Dahee Kim, Yuri Kim, Jinmyoung Joo, Bong Hoon Kim, Yangzhi Zhu, Sung Gyu Park, Hyun Cheol SongWujin Sun, Jae Pyoung Ahn, Woo Young Jang, Ramasamy Paulmurugan, Hong Kyu Kim, Jong Seung Kim, Heemin Kang

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Extracellular matrix (ECM) proteins containing cell-attachable Arg-Gly-Asp (RGD) sequences exhibit variable bridging and non-bridging in fibronectin-collagen and laminin-collagen complexes that can regulate inflammation, tissue repair, and wound healing. In this study, linking molecule-mediated conjugation of 1D magnetic nanocylinders (MNCs) to material surfaces pre-decorated with gold nanospheres (GNSs) is performed, thereby yielding RGD-coated MNCs (RGD-MNCs) over RGD-coated GNSs (RGD-GNSs) in a non-bridging state. The RGD-MNCs are drawn closer to the RGD-GNSs via magnetic field-mediated compression of the linking molecules to establish the bridging between them. Relative proportion of the RGD-MNCs to the RGD-GNSs is optimized to yield effective remote stimulation of integrin binding to variably bridged RGDs similar to that of invariably bridged RGDs used as a control group. Remote manipulation of the RGD bridging facilitates the attachment structure assembly of macrophages that leads to pro-healing/anti-inflammatory phenotype acquisition. In contrast, the non-bridged RGDs inhibited macrophage attachment that acquired pro-inflammatory phenotypes. The use of various nanomaterials in constructing heterogeneous RGD-coated materials can further offer various modes in remote switching of RGD bridging and non-bridging to understand dynamic integrin-mediated modulation of macrophages that regulate immunomodulatory responses, such as foreign body responses, tissue repair, and wound healing.

Original languageEnglish
Article number2215166
JournalAdvanced Functional Materials
Volume33
Issue number16
DOIs
Publication statusPublished - 2023 Apr 18

Bibliographical note

Publisher Copyright:
© 2023 Wiley-VCH GmbH.

Keywords

  • in situ bridging
  • ligand bridging
  • macrophage adhesions
  • macrophage polarization
  • remote controls

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • General Chemistry
  • Condensed Matter Physics
  • General Materials Science
  • Electrochemistry
  • Biomaterials

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