Molecular and histologic evidence of novel erectile dysfunction rat model as an aging atherosclerosis model: A preliminary study

Jae Heon Kim, Ji Sung Shim, Jong Wook Kim, Seung Whan Doo, Jae Hyun Bae, Ju Han Lee, Yun Seob Song, Je Jong Kim, Du Geon Moon

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    1 Citation (Scopus)


    Purpose: To validate a novel arteriogenic erectile dysfunction (ED) model with atherosclerosis (AS) based on molecular and histologic evidence induced by chronic pelvic ischemia (CPI) and determine effect of phosphodiesterase-5 inhibitor treatment. Materials and Methods: Twenty 16-week-old male Sprague–Dawley rats were divided into three experimental groups (Group I, untreated sham-operated rats with regular diet; Group II, CPI with cholesterol diet; Group III, CPI model with cholesterol diet and mirodenafil). Erectile function was accessed using maximum intracavernous pressure (ICP) and ICP/mean arterial pressure (MAP). Molecular changes were examined by western blot analysis using hypoxia inducible factor 1-alpha (HIF-1α), endothelial nitric oxide synthase (eNOS), and transforming growth factor beta-1 (TGF-β1) antibodies. Collagen change was evaluated by Masson’s trichrome staining. Results: In vivo measurements of ICP and ICP/MAP in Group II were significantly lower than those in Group I (p<0.01). Smooth muscle/collagen ratio in Group II was significantly lower than that in Group I (p<0.05). After treatment with mirodenafil for four weeks, Group III showed significantly higher levels of ICP and ICP/MAP than Group II (p<0.05). Western blot analysis showed that HIF-1α and TGF-β1 levels were significantly higher in Group II whereas eNOS levels were significantly lower in Group II than those in Group I or III. Conclusions: A novel arteriogenic ED with AS model is successfully induced by CPI and validated based on molecular and histologic evidences.

    Original languageEnglish
    JournalWorld Journal of Men?s Health
    Publication statusPublished - 2019


    • Atherosclerosis
    • Erectile dysfunction
    • Phosphodiesterase 5 inhibitors
    • Vasculogenic impotence

    ASJC Scopus subject areas

    • Ageing
    • Reproductive Medicine
    • Health Policy
    • Public Health, Environmental and Occupational Health
    • Psychiatry and Mental health
    • Urology
    • Pharmacology (medical)


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