TY - JOUR
T1 - Molecular characterization of high-level gentamicin-resistant Enterococcus faecalis from chicken meat in Korea
AU - Choi, Jong Mi
AU - Woo, Gun Jo
N1 - Funding Information:
This study was supported by grants from the National Antimicrobial Resistance Management Program (NARMP) of the Korean Food and Drug Administration . We thank the Korea University Food Safety Hall and Institute of Food and Biomedicine Safety for allowing the use of their equipments and facilities.
PY - 2013/7/1
Y1 - 2013/7/1
N2 - Because the intrinsically antimicrobial-resistant Enterococcus has acquired high-level aminoglycoside resistance genes, treating enterococcal infections is difficult. In this study, of the 101 food-borne Enterococcus faecalis isolates collected from retail chicken meat between 2003 and 2010, 11 high-level gentamicin-resistant (HLGR) E. faecalis isolates (MICs. > 2,048. μg/mL) were found. Molecular characterization was performed to determine the basis of this resistance. All HLGR E. faecalis isolates encoded aac(6')- Ie- aph(2")-. Ia and harbored at least 3 virulence traits in the asa1, esp, gelE, efaA, ace, and cylA genes. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were performed to characterize their molecular epidemiology. A total of 8 sequence types (STs), including 3 novel STs, were identified (ST35, ST82, ST116, ST202, ST300, ST403, ST407, and ST420). ST82, which is associated with amyloid arthropathy in poultry, was the most prevalent ST among HLGR E. faecalis isolates (4 out of 11 isolates, 36.4%); all other STs were identified in the isolates as well. The STs of food-borne HLGR E. faecalis in this study have been confirmed as corresponding to clinical isolates in the MLST database (DB), except for ST300 and the new STs. Three out of 11 isolates belonged to CC116, including ST116, ST407, and ST420. This study characterized HLGR E. faecalis isolates and provided evidence for the spread of HLGR E. faecalis with virulence factors to chicken sources in Korea. The emergence of food-borne HLGR E. faecalis suggests that chicken could be a potential source of transmission of antimicrobial resistance and virulence factors.
AB - Because the intrinsically antimicrobial-resistant Enterococcus has acquired high-level aminoglycoside resistance genes, treating enterococcal infections is difficult. In this study, of the 101 food-borne Enterococcus faecalis isolates collected from retail chicken meat between 2003 and 2010, 11 high-level gentamicin-resistant (HLGR) E. faecalis isolates (MICs. > 2,048. μg/mL) were found. Molecular characterization was performed to determine the basis of this resistance. All HLGR E. faecalis isolates encoded aac(6')- Ie- aph(2")-. Ia and harbored at least 3 virulence traits in the asa1, esp, gelE, efaA, ace, and cylA genes. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were performed to characterize their molecular epidemiology. A total of 8 sequence types (STs), including 3 novel STs, were identified (ST35, ST82, ST116, ST202, ST300, ST403, ST407, and ST420). ST82, which is associated with amyloid arthropathy in poultry, was the most prevalent ST among HLGR E. faecalis isolates (4 out of 11 isolates, 36.4%); all other STs were identified in the isolates as well. The STs of food-borne HLGR E. faecalis in this study have been confirmed as corresponding to clinical isolates in the MLST database (DB), except for ST300 and the new STs. Three out of 11 isolates belonged to CC116, including ST116, ST407, and ST420. This study characterized HLGR E. faecalis isolates and provided evidence for the spread of HLGR E. faecalis with virulence factors to chicken sources in Korea. The emergence of food-borne HLGR E. faecalis suggests that chicken could be a potential source of transmission of antimicrobial resistance and virulence factors.
KW - Aac(6')-Ie-aph(2")-Ia
KW - Food-borne Enterococcus faecalis
KW - High-level gentamicin resistance
KW - MLST
KW - PFGE
UR - http://www.scopus.com/inward/record.url?scp=84877908448&partnerID=8YFLogxK
U2 - 10.1016/j.ijfoodmicro.2013.02.016
DO - 10.1016/j.ijfoodmicro.2013.02.016
M3 - Article
C2 - 23685465
AN - SCOPUS:84877908448
SN - 0168-1605
VL - 165
SP - 1
EP - 6
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
IS - 1
ER -