Molecular characterization of the AtCXE8 gene, which promotes resistance to Botrytis cinerea infection

Sanghyeob Lee; Seongbin Hwang; Yong Weon Seo; Woong Bae Jeon; Boung Jun Oh

doi:10.1007/s11816-012-0253-0

Molecular characterization of the AtCXE8 gene, which promotes resistance to Botrytis cinerea infection

Sanghyeob Lee
, Seongbin Hwang
, Yong Weon Seo
, Woong Bae Jeon
, Boung Jun Oh

Department of Biosystems and Biotechnology

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The relationships of sterase- and lipase-like proteins with three signaling molecules (salicylic acid, jasmonic acid, and ethylene) expressed during plant-pathogen interactions were studied. We isolated two carboxylesterase (AtCXE) genes, AtCXE8 and AtCXE9, from Arabidopsis thaliana. The AtCXE8 and AtCXE9 proteins possess carboxylesterase motifs (-GXSXG-) and catalytic triads (Ser, Asp, and His). We demonstrated that recombinant AtCXE8 and AtCXE9 proteins have both enzymatic activity and specific activity for p-nitrophenyl butyrate (C₄) in vitro. Moreover, the enzymatic activity of recombinant AtCXE8 was twofold higher than that of AtCXE9. To gain a better understanding of the endogenous role of the AtCXE8 gene in Arabidopsis, we identified an enhancer trap T-DNA mutant (AtCXE8_KO) and used it to show that the AtCXE8 gene was induced in response to fungal infection. AtCXE8_KO plants were also more susceptible to infections than wild-type Col-0 plants. Moreover, overexpression of the AtCXE8 gene in transgenic Arabidopsis plants led to enhanced disease resistance against B. cinerea. Taken together, our data indicate that AtCXE8 plays a role in promoting resistance to fungal invasion.

Original language	English
Pages (from-to)	109-119
Number of pages	11
Journal	Plant Biotechnology Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1007/s11816-012-0253-0
Publication status	Published - 2013 Jan

Bibliographical note

Funding Information:
Acknowledgments We thank the Arabidopsis Biological Research Center, The Arabidopsis Information Resource, and The University of Wisconsin Biotechnology Center for providing Arabidopsis thaliana Col-0, Col-6 ecotypes, ein2-1 mutant, and enhancer trap T-DNA mutants, respectively. We also thank Willem F. Broekaert (Kath-olieke Universiteit Leuven) for providing A. brassicicola strain IMI169558. This work was supported by the faculty research fund of Sejong University in 2011, the Bio-Industry Technology Development Program of iPET (111057-5), and the National Research Foundation of Korea (NRF) of the Korean government (MEST) (no. 2011-0001105) for S. Lee, and a Korea University Grant for W. Jeon.

Keywords

Abiotic stress
Botrytis cinerea
Carboxylesterase
Disease resistance

ASJC Scopus subject areas

Biotechnology
Plant Science

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10.1007/s11816-012-0253-0

Cite this

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title = "Molecular characterization of the AtCXE8 gene, which promotes resistance to Botrytis cinerea infection",

abstract = "The relationships of sterase- and lipase-like proteins with three signaling molecules (salicylic acid, jasmonic acid, and ethylene) expressed during plant-pathogen interactions were studied. We isolated two carboxylesterase (AtCXE) genes, AtCXE8 and AtCXE9, from Arabidopsis thaliana. The AtCXE8 and AtCXE9 proteins possess carboxylesterase motifs (-GXSXG-) and catalytic triads (Ser, Asp, and His). We demonstrated that recombinant AtCXE8 and AtCXE9 proteins have both enzymatic activity and specific activity for p-nitrophenyl butyrate (C4) in vitro. Moreover, the enzymatic activity of recombinant AtCXE8 was twofold higher than that of AtCXE9. To gain a better understanding of the endogenous role of the AtCXE8 gene in Arabidopsis, we identified an enhancer trap T-DNA mutant (AtCXE8\_KO) and used it to show that the AtCXE8 gene was induced in response to fungal infection. AtCXE8\_KO plants were also more susceptible to infections than wild-type Col-0 plants. Moreover, overexpression of the AtCXE8 gene in transgenic Arabidopsis plants led to enhanced disease resistance against B. cinerea. Taken together, our data indicate that AtCXE8 plays a role in promoting resistance to fungal invasion.",

keywords = "Abiotic stress, Botrytis cinerea, Carboxylesterase, Disease resistance",

author = "Sanghyeob Lee and Seongbin Hwang and Seo, \{Yong Weon\} and Jeon, \{Woong Bae\} and Oh, \{Boung Jun\}",

note = "Funding Information: Acknowledgments We thank the Arabidopsis Biological Research Center, The Arabidopsis Information Resource, and The University of Wisconsin Biotechnology Center for providing Arabidopsis thaliana Col-0, Col-6 ecotypes, ein2-1 mutant, and enhancer trap T-DNA mutants, respectively. We also thank Willem F. Broekaert (Kath-olieke Universiteit Leuven) for providing A. brassicicola strain IMI169558. This work was supported by the faculty research fund of Sejong University in 2011, the Bio-Industry Technology Development Program of iPET (111057-5), and the National Research Foundation of Korea (NRF) of the Korean government (MEST) (no. 2011-0001105) for S. Lee, and a Korea University Grant for W. Jeon.",

year = "2013",

month = jan,

doi = "10.1007/s11816-012-0253-0",

language = "English",

volume = "7",

pages = "109--119",

journal = "Plant Biotechnology Reports",

issn = "1863-5466",

publisher = "Springer",

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T1 - Molecular characterization of the AtCXE8 gene, which promotes resistance to Botrytis cinerea infection

AU - Lee, Sanghyeob

AU - Hwang, Seongbin

AU - Seo, Yong Weon

AU - Jeon, Woong Bae

AU - Oh, Boung Jun

N1 - Funding Information: Acknowledgments We thank the Arabidopsis Biological Research Center, The Arabidopsis Information Resource, and The University of Wisconsin Biotechnology Center for providing Arabidopsis thaliana Col-0, Col-6 ecotypes, ein2-1 mutant, and enhancer trap T-DNA mutants, respectively. We also thank Willem F. Broekaert (Kath-olieke Universiteit Leuven) for providing A. brassicicola strain IMI169558. This work was supported by the faculty research fund of Sejong University in 2011, the Bio-Industry Technology Development Program of iPET (111057-5), and the National Research Foundation of Korea (NRF) of the Korean government (MEST) (no. 2011-0001105) for S. Lee, and a Korea University Grant for W. Jeon.

PY - 2013/1

Y1 - 2013/1

N2 - The relationships of sterase- and lipase-like proteins with three signaling molecules (salicylic acid, jasmonic acid, and ethylene) expressed during plant-pathogen interactions were studied. We isolated two carboxylesterase (AtCXE) genes, AtCXE8 and AtCXE9, from Arabidopsis thaliana. The AtCXE8 and AtCXE9 proteins possess carboxylesterase motifs (-GXSXG-) and catalytic triads (Ser, Asp, and His). We demonstrated that recombinant AtCXE8 and AtCXE9 proteins have both enzymatic activity and specific activity for p-nitrophenyl butyrate (C4) in vitro. Moreover, the enzymatic activity of recombinant AtCXE8 was twofold higher than that of AtCXE9. To gain a better understanding of the endogenous role of the AtCXE8 gene in Arabidopsis, we identified an enhancer trap T-DNA mutant (AtCXE8_KO) and used it to show that the AtCXE8 gene was induced in response to fungal infection. AtCXE8_KO plants were also more susceptible to infections than wild-type Col-0 plants. Moreover, overexpression of the AtCXE8 gene in transgenic Arabidopsis plants led to enhanced disease resistance against B. cinerea. Taken together, our data indicate that AtCXE8 plays a role in promoting resistance to fungal invasion.

AB - The relationships of sterase- and lipase-like proteins with three signaling molecules (salicylic acid, jasmonic acid, and ethylene) expressed during plant-pathogen interactions were studied. We isolated two carboxylesterase (AtCXE) genes, AtCXE8 and AtCXE9, from Arabidopsis thaliana. The AtCXE8 and AtCXE9 proteins possess carboxylesterase motifs (-GXSXG-) and catalytic triads (Ser, Asp, and His). We demonstrated that recombinant AtCXE8 and AtCXE9 proteins have both enzymatic activity and specific activity for p-nitrophenyl butyrate (C4) in vitro. Moreover, the enzymatic activity of recombinant AtCXE8 was twofold higher than that of AtCXE9. To gain a better understanding of the endogenous role of the AtCXE8 gene in Arabidopsis, we identified an enhancer trap T-DNA mutant (AtCXE8_KO) and used it to show that the AtCXE8 gene was induced in response to fungal infection. AtCXE8_KO plants were also more susceptible to infections than wild-type Col-0 plants. Moreover, overexpression of the AtCXE8 gene in transgenic Arabidopsis plants led to enhanced disease resistance against B. cinerea. Taken together, our data indicate that AtCXE8 plays a role in promoting resistance to fungal invasion.

KW - Abiotic stress

KW - Botrytis cinerea

KW - Carboxylesterase

KW - Disease resistance

UR - https://www.scopus.com/pages/publications/84872319154

U2 - 10.1007/s11816-012-0253-0

DO - 10.1007/s11816-012-0253-0

M3 - Article

AN - SCOPUS:84872319154

SN - 1863-5466

VL - 7

SP - 109

EP - 119

JO - Plant Biotechnology Reports

JF - Plant Biotechnology Reports

IS - 1

ER -

Molecular characterization of the AtCXE8 gene, which promotes resistance to Botrytis cinerea infection

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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