Molecular characterization of wheat floret development-related F-box protein (TaF-box2): Possible involvement in regulation of Arabidopsis flowering

Jae Ho Kim, Woo Joo Jung, Moon Seok Kim, Chan Seop Ko, Jin Seok Yoon, Min Jeong Hong, Hyo Jeong Shin, Yong Weon Seo

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


In wheat (Triticum aestivum L.), the floret development stage is an important step in determining grain yield per spike; however, the molecular mechanisms underlying floret development remain unclear. In this study, we elucidated the role of TaF-box2, a member of the F-box-containing E3 ubiquitin protein ligases, which is involved in floret development and anthesis of wheat. TaF-box2 was transiently expressed in the plasma membrane and cytoplasm of both tobacco and wheat. We also found that the SCFF-box2(Skp1-Cul1-Rbx1-TaF-box2) ubiquitin ligase complex mediated self-ubiquitination activity. Transgenic Arabidopsis plants that constitutively overexpressed TaF-box2 showed markedly greater hypocotyl and root length than wild-type plants, and produced early flowering phenotypes. Flowering-related genes were significantly upregulated in TaF-box2-overexpressing Arabidopsis plants. Further protein interaction analyses such as yeast two-hybrid, in vitro pull-down, and bimolecular fluorescence complementation assays confirmed that TaF-box2 physically interacted with TaCYCL1 (Triticum aestivum cyclin-L1-1). Ubiquitination and degradation assays demonstrated that TaCYCL1 was ubiquitinated by SCFF-box2 and degraded through the 26S proteasome complex. The physiological functions of the TaF-box2 protein remain unclear; however, we discuss several potential routes of involvement in various physiological mechanisms which counteract flowering in transgenic Arabidopsis plants.

Original languageEnglish
Article numbere13677
JournalPhysiologia Plantarum
Issue number2
Publication statusPublished - 2022 Mar 1

Bibliographical note

Funding Information:
This work was supported by a grant from the Korea University and Basic Science Research Program through the National Research Foundation of Korea, which is funded by the Ministry of Science, ICT & Future Planning (grant number 2017R1D1A1B06030349).

Publisher Copyright:
© 2022 Scandinavian Plant Physiology Society.

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology


Dive into the research topics of 'Molecular characterization of wheat floret development-related F-box protein (TaF-box2): Possible involvement in regulation of Arabidopsis flowering'. Together they form a unique fingerprint.

Cite this