Molecular cloning and expression analysis of CD82 in pig

Kyu Won Cho; Jong Gun Kim; Kwang Youn Whang; Woon Won Jung; Young Bong Kim

doi:10.1080/10495390802292363

Molecular cloning and expression analysis of CD82 in pig

Kyu Won Cho, Jong Gun Kim, Kwang Youn Whang, Woon Won Jung, Young Bong Kim

Research output: Contribution to journal › Article › peer-review

Abstract

CD82, which was originally referred to as KAI1 (kangai 1), is a member of the tetraspanin protein family, which contains four transmembrane domains. CD82 is implicated in a variety of biological processes, including apoptosis, cell adhesion, and cell migration. In this study, the full-length cDNA of pig CD82 was cloned and sequenced. Pig Cd82 cDNA contains an open reading frame (801 bp) encoding 266 amino acids. Sequence alignment results indicated that pig CD82 cDNA evidenced 85.45%, 85.63%, 77.03%, and 77.78% identity with human, cattle, rat, and mouse, respectively. In the expression study, the constitutive expression of swine Cd82 mRNA was detected in a variety of tissues, including lymphoid tissues as well as nonlymphoid tissues. Future studies will be focused on the functional role of CD82 during the course of pig infectious diseases or tumor development.

Original language	English
Pages (from-to)	237-242
Number of pages	6
Journal	Animal Biotechnology
Volume	19
Issue number	4
DOIs	https://doi.org/10.1080/10495390802292363
Publication status	Published - 2008 Oct

Keywords

CD82
Inflammation
Lipopolysaccharide
Pig
Tetraspanin protein family

ASJC Scopus subject areas

Biotechnology
Bioengineering
Animal Science and Zoology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1080/10495390802292363

Cite this

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title = "Molecular cloning and expression analysis of CD82 in pig",

abstract = "CD82, which was originally referred to as KAI1 (kangai 1), is a member of the tetraspanin protein family, which contains four transmembrane domains. CD82 is implicated in a variety of biological processes, including apoptosis, cell adhesion, and cell migration. In this study, the full-length cDNA of pig CD82 was cloned and sequenced. Pig Cd82 cDNA contains an open reading frame (801 bp) encoding 266 amino acids. Sequence alignment results indicated that pig CD82 cDNA evidenced 85.45%, 85.63%, 77.03%, and 77.78% identity with human, cattle, rat, and mouse, respectively. In the expression study, the constitutive expression of swine Cd82 mRNA was detected in a variety of tissues, including lymphoid tissues as well as nonlymphoid tissues. Future studies will be focused on the functional role of CD82 during the course of pig infectious diseases or tumor development.",

keywords = "CD82, Inflammation, Lipopolysaccharide, Pig, Tetraspanin protein family",

author = "Cho, {Kyu Won} and Kim, {Jong Gun} and Whang, {Kwang Youn} and Jung, {Woon Won} and Kim, {Young Bong}",

year = "2008",

month = oct,

doi = "10.1080/10495390802292363",

language = "English",

volume = "19",

pages = "237--242",

journal = "Animal Biotechnology",

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TY - JOUR

T1 - Molecular cloning and expression analysis of CD82 in pig

AU - Cho, Kyu Won

AU - Kim, Jong Gun

AU - Whang, Kwang Youn

AU - Jung, Woon Won

AU - Kim, Young Bong

PY - 2008/10

Y1 - 2008/10

N2 - CD82, which was originally referred to as KAI1 (kangai 1), is a member of the tetraspanin protein family, which contains four transmembrane domains. CD82 is implicated in a variety of biological processes, including apoptosis, cell adhesion, and cell migration. In this study, the full-length cDNA of pig CD82 was cloned and sequenced. Pig Cd82 cDNA contains an open reading frame (801 bp) encoding 266 amino acids. Sequence alignment results indicated that pig CD82 cDNA evidenced 85.45%, 85.63%, 77.03%, and 77.78% identity with human, cattle, rat, and mouse, respectively. In the expression study, the constitutive expression of swine Cd82 mRNA was detected in a variety of tissues, including lymphoid tissues as well as nonlymphoid tissues. Future studies will be focused on the functional role of CD82 during the course of pig infectious diseases or tumor development.

AB - CD82, which was originally referred to as KAI1 (kangai 1), is a member of the tetraspanin protein family, which contains four transmembrane domains. CD82 is implicated in a variety of biological processes, including apoptosis, cell adhesion, and cell migration. In this study, the full-length cDNA of pig CD82 was cloned and sequenced. Pig Cd82 cDNA contains an open reading frame (801 bp) encoding 266 amino acids. Sequence alignment results indicated that pig CD82 cDNA evidenced 85.45%, 85.63%, 77.03%, and 77.78% identity with human, cattle, rat, and mouse, respectively. In the expression study, the constitutive expression of swine Cd82 mRNA was detected in a variety of tissues, including lymphoid tissues as well as nonlymphoid tissues. Future studies will be focused on the functional role of CD82 during the course of pig infectious diseases or tumor development.

KW - CD82

KW - Inflammation

KW - Lipopolysaccharide

KW - Pig

KW - Tetraspanin protein family

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SN - 1049-5398

VL - 19

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EP - 242

JO - Animal Biotechnology

JF - Animal Biotechnology

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ER -

Molecular cloning and expression analysis of CD82 in pig

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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