Molecular cloning and expression analysis of pig CD138

Joonbeom Bae; Seonah Jeong; Ju Yeon Lee; Hyun Jeong Lee; Bong Hwan Choi; Ji Eun Kim; Inho Choi; Taehoon Chun

doi:10.1016/j.rvsc.2013.09.010

Molecular cloning and expression analysis of pig CD138

Joonbeom Bae, Seonah Jeong, Ju Yeon Lee, Hyun Jeong Lee, Bong Hwan Choi, Ji Eun Kim, Inho Choi, Taehoon Chun

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

Abstract

CD138 (syndecan-1) interacts with various components of the extracellular matrix and associates with the actin cytoskeleton. In this study, we cloned pig CD138 cDNA and determined its complete cDNA sequence. Pig CD138 cDNA contained an open reading frame (930. bp) encoding 309 amino acids with five well conserved putative glycosaminoglycan attachment sites, a putative cleavage site for matrix metalloproteinases, and conserved motifs involved in signal transduction among mammalian species. Pig CD138 mRNA was detected in various tissues, including lymphoid and non-lymphoid organs, indicating the multicellular functions of CD138 in pigs. Western blot and flow cytometry analyses detected an approximate 35. kDa pig CD138 protein expressed on the cell surface. Further immunohistochemistry analysis revealed that CD138 expression was mainly observed in submucosa and lamina propria of the pig small intestine. Further study will be necessary to define the functional importance of CD138 during specific infectious diseases in pigs.

Original language	English
Pages (from-to)	1021-1025
Number of pages	5
Journal	Research in Veterinary Science
Volume	95
Issue number	3
DOIs	https://doi.org/10.1016/j.rvsc.2013.09.010
Publication status	Published - 2013 Dec

Keywords

CD138
CDNA cloning
Expression
Pig
Syndecan

ASJC Scopus subject areas

veterinary(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.rvsc.2013.09.010

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@article{35da46c22ede464fa8c082d191ec494a,

title = "Molecular cloning and expression analysis of pig CD138",

abstract = "CD138 (syndecan-1) interacts with various components of the extracellular matrix and associates with the actin cytoskeleton. In this study, we cloned pig CD138 cDNA and determined its complete cDNA sequence. Pig CD138 cDNA contained an open reading frame (930. bp) encoding 309 amino acids with five well conserved putative glycosaminoglycan attachment sites, a putative cleavage site for matrix metalloproteinases, and conserved motifs involved in signal transduction among mammalian species. Pig CD138 mRNA was detected in various tissues, including lymphoid and non-lymphoid organs, indicating the multicellular functions of CD138 in pigs. Western blot and flow cytometry analyses detected an approximate 35. kDa pig CD138 protein expressed on the cell surface. Further immunohistochemistry analysis revealed that CD138 expression was mainly observed in submucosa and lamina propria of the pig small intestine. Further study will be necessary to define the functional importance of CD138 during specific infectious diseases in pigs.",

keywords = "CD138, CDNA cloning, Expression, Pig, Syndecan",

author = "Joonbeom Bae and Seonah Jeong and Lee, {Ju Yeon} and Lee, {Hyun Jeong} and Choi, {Bong Hwan} and Kim, {Ji Eun} and Inho Choi and Taehoon Chun",

note = "Funding Information: This study was supported by a grant from the Research Cooperating Program for Agricultural Science & Technology Development (project No. PJ006406 ) and Next-Generation BioGreen 21 program (No. PJ008089 ), Rural Development Administration, Republic of Korea. ",

year = "2013",

month = dec,

doi = "10.1016/j.rvsc.2013.09.010",

language = "English",

volume = "95",

pages = "1021--1025",

journal = "Research in Veterinary Science",

issn = "0034-5288",

publisher = "Elsevier",

number = "3",

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T1 - Molecular cloning and expression analysis of pig CD138

AU - Bae, Joonbeom

AU - Jeong, Seonah

AU - Lee, Ju Yeon

AU - Lee, Hyun Jeong

AU - Choi, Bong Hwan

AU - Kim, Ji Eun

AU - Choi, Inho

AU - Chun, Taehoon

N1 - Funding Information: This study was supported by a grant from the Research Cooperating Program for Agricultural Science & Technology Development (project No. PJ006406 ) and Next-Generation BioGreen 21 program (No. PJ008089 ), Rural Development Administration, Republic of Korea.

PY - 2013/12

Y1 - 2013/12

N2 - CD138 (syndecan-1) interacts with various components of the extracellular matrix and associates with the actin cytoskeleton. In this study, we cloned pig CD138 cDNA and determined its complete cDNA sequence. Pig CD138 cDNA contained an open reading frame (930. bp) encoding 309 amino acids with five well conserved putative glycosaminoglycan attachment sites, a putative cleavage site for matrix metalloproteinases, and conserved motifs involved in signal transduction among mammalian species. Pig CD138 mRNA was detected in various tissues, including lymphoid and non-lymphoid organs, indicating the multicellular functions of CD138 in pigs. Western blot and flow cytometry analyses detected an approximate 35. kDa pig CD138 protein expressed on the cell surface. Further immunohistochemistry analysis revealed that CD138 expression was mainly observed in submucosa and lamina propria of the pig small intestine. Further study will be necessary to define the functional importance of CD138 during specific infectious diseases in pigs.

AB - CD138 (syndecan-1) interacts with various components of the extracellular matrix and associates with the actin cytoskeleton. In this study, we cloned pig CD138 cDNA and determined its complete cDNA sequence. Pig CD138 cDNA contained an open reading frame (930. bp) encoding 309 amino acids with five well conserved putative glycosaminoglycan attachment sites, a putative cleavage site for matrix metalloproteinases, and conserved motifs involved in signal transduction among mammalian species. Pig CD138 mRNA was detected in various tissues, including lymphoid and non-lymphoid organs, indicating the multicellular functions of CD138 in pigs. Western blot and flow cytometry analyses detected an approximate 35. kDa pig CD138 protein expressed on the cell surface. Further immunohistochemistry analysis revealed that CD138 expression was mainly observed in submucosa and lamina propria of the pig small intestine. Further study will be necessary to define the functional importance of CD138 during specific infectious diseases in pigs.

KW - CD138

KW - CDNA cloning

KW - Expression

KW - Pig

KW - Syndecan

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U2 - 10.1016/j.rvsc.2013.09.010

DO - 10.1016/j.rvsc.2013.09.010

M3 - Article

C2 - 24128845

AN - SCOPUS:84887988252

SN - 0034-5288

VL - 95

SP - 1021

EP - 1025

JO - Research in Veterinary Science

JF - Research in Veterinary Science

IS - 3

ER -

Molecular cloning and expression analysis of pig CD138

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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