Molecular cloning and expression analysis of pig CD7

Ju Yeon Lee, Joonbeom Bae, Inho Choi, Chung Gyu Park, Taehoon Chun

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

CD7 is an integral membrane protein which mediates an important signal to mediate the differentiation, activation, and regulation of some T cells and NK cells. However, only human and mouse CD7 have been identified and studied among mammalian species. In this study, we cloned pig CD7 cDNA and determined its complete cDNA sequence. Pig CD7 cDNA contained an open reading frame (627 bp) encoding 208 amino acids with well conserved motifs involved in signal transduction within cytoplasmic tail among mammalian species. Pig CD7 mRNA was detected by RT-PCR in mainly lymphoid tissues, indicating the conserved functions of CD7 in pigs. Moreover, we generated soluble pig CD7 fusion immunoglobulin (pig CD7Ig) containing extracellular domain of pig CD7 to test whether pig CD7 binds to pig galectin-3. Flow cytometry and immunohistochemistry analyses indicated that soluble pig CD7Ig can bind to galectin-3 expressed in macrophages and epithelial cells of small intestine. These results help to analyze the structural relationship between CD7 and its ligand transferring signal transduction among mammalian species.

Original languageEnglish
Pages (from-to)257-263
Number of pages7
JournalVeterinary Research Communications
Volume38
Issue number3
DOIs
Publication statusPublished - 2014 Sept

Bibliographical note

Funding Information:
Acknowledgments This work was supported by a grant from the Next-Generation BioGreen 21 program (No. PJ008089), Rural Development Administration, Republic of Korea and a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health &Welfare, Republic of Korea (grant number : HI13C0954).

Keywords

  • CD7
  • Galectin-3
  • Pig
  • Soluble fusion protein
  • cDNA

ASJC Scopus subject areas

  • General Veterinary

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