Molecular Cloning and Expression Analysis of Pig Cd90

Kyungmin Park; Jonggun Kim; Chang Yong Choi; Joonbeom Bae; Sang Hoon Kim; Yeon Hui Kim; Taehoon Chun

doi:10.1080/10495398.2015.1129630

Molecular Cloning and Expression Analysis of Pig Cd90

Kyungmin Park, Jonggun Kim, Chang Yong Choi, Joonbeom Bae, Sang Hoon Kim, Yeon Hui Kim, Taehoon Chun

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

ABSTRACT: The CD90 (Thy-1) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that transfers signals involved in many biological events including cell activation, cell migration, cell adhesion, and tumor suppression. In this study, we cloned pig CD90 cDNA and determined its complete cDNA sequence. Pig CD90 cDNA contained an open reading frame (486 bp) encoding 161 amino acids with three putative N-glycosylation sites and four well-conserved cysteine residues, which form a possible disulfide bond within the extracellular domain among mammalian species. Pig CD90 mRNA was detected in various tissues, indicating the multicellular functions of CD90 in pigs. Flow cytometry analyses demonstrated that anti-human CD90 antibody recognizes a pig CD90 on the cell surface. Moreover, immunohistochemistry analysis revealed that CD90 expression is widely diffused in several pig tissues. Further studies will be necessary to define the functional contribution of CD90 during specific infectious diseases in pigs.

Original language	English
Pages (from-to)	133-139
Number of pages	7
Journal	Animal Biotechnology
Volume	27
Issue number	2
DOIs	https://doi.org/10.1080/10495398.2015.1129630
Publication status	Published - 2016 Apr 2

Bibliographical note

Funding Information:
This work was supported by a grant from the Next-Generation BioGreen 21 program (Project No. PJ01113001), Rural Development Administration, Republic of Korea and a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health &Welfare, Republic of Korea (Grant No. HI13C0954).

Keywords

CD90
cDNA cloning
expression
glycosylphosphatidylinositol (GPI)-anchored glycoprotein
pig

ASJC Scopus subject areas

Biotechnology
Bioengineering
Animal Science and Zoology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1080/10495398.2015.1129630

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abstract = "ABSTRACT: The CD90 (Thy-1) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that transfers signals involved in many biological events including cell activation, cell migration, cell adhesion, and tumor suppression. In this study, we cloned pig CD90 cDNA and determined its complete cDNA sequence. Pig CD90 cDNA contained an open reading frame (486 bp) encoding 161 amino acids with three putative N-glycosylation sites and four well-conserved cysteine residues, which form a possible disulfide bond within the extracellular domain among mammalian species. Pig CD90 mRNA was detected in various tissues, indicating the multicellular functions of CD90 in pigs. Flow cytometry analyses demonstrated that anti-human CD90 antibody recognizes a pig CD90 on the cell surface. Moreover, immunohistochemistry analysis revealed that CD90 expression is widely diffused in several pig tissues. Further studies will be necessary to define the functional contribution of CD90 during specific infectious diseases in pigs.",

keywords = "CD90, cDNA cloning, expression, glycosylphosphatidylinositol (GPI)-anchored glycoprotein, pig",

author = "Kyungmin Park and Jonggun Kim and Choi, {Chang Yong} and Joonbeom Bae and Kim, {Sang Hoon} and Kim, {Yeon Hui} and Taehoon Chun",

note = "Funding Information: This work was supported by a grant from the Next-Generation BioGreen 21 program (Project No. PJ01113001), Rural Development Administration, Republic of Korea and a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health &Welfare, Republic of Korea (Grant No. HI13C0954).",

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AU - Park, Kyungmin

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AU - Choi, Chang Yong

AU - Bae, Joonbeom

AU - Kim, Sang Hoon

AU - Kim, Yeon Hui

AU - Chun, Taehoon

N1 - Funding Information: This work was supported by a grant from the Next-Generation BioGreen 21 program (Project No. PJ01113001), Rural Development Administration, Republic of Korea and a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health &Welfare, Republic of Korea (Grant No. HI13C0954).

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Y1 - 2016/4/2

N2 - ABSTRACT: The CD90 (Thy-1) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that transfers signals involved in many biological events including cell activation, cell migration, cell adhesion, and tumor suppression. In this study, we cloned pig CD90 cDNA and determined its complete cDNA sequence. Pig CD90 cDNA contained an open reading frame (486 bp) encoding 161 amino acids with three putative N-glycosylation sites and four well-conserved cysteine residues, which form a possible disulfide bond within the extracellular domain among mammalian species. Pig CD90 mRNA was detected in various tissues, indicating the multicellular functions of CD90 in pigs. Flow cytometry analyses demonstrated that anti-human CD90 antibody recognizes a pig CD90 on the cell surface. Moreover, immunohistochemistry analysis revealed that CD90 expression is widely diffused in several pig tissues. Further studies will be necessary to define the functional contribution of CD90 during specific infectious diseases in pigs.

AB - ABSTRACT: The CD90 (Thy-1) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that transfers signals involved in many biological events including cell activation, cell migration, cell adhesion, and tumor suppression. In this study, we cloned pig CD90 cDNA and determined its complete cDNA sequence. Pig CD90 cDNA contained an open reading frame (486 bp) encoding 161 amino acids with three putative N-glycosylation sites and four well-conserved cysteine residues, which form a possible disulfide bond within the extracellular domain among mammalian species. Pig CD90 mRNA was detected in various tissues, indicating the multicellular functions of CD90 in pigs. Flow cytometry analyses demonstrated that anti-human CD90 antibody recognizes a pig CD90 on the cell surface. Moreover, immunohistochemistry analysis revealed that CD90 expression is widely diffused in several pig tissues. Further studies will be necessary to define the functional contribution of CD90 during specific infectious diseases in pigs.

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Molecular Cloning and Expression Analysis of Pig Cd90

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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