Lymphocyte activation gene-3 (LAG-3; CD223) is a co-receptor that may function as a down-modulator of T cell activity via an interaction with MHC class II molecules. In this study, we cloned and sequenced the complete cDNA sequence of pig Lag3. Pig Lag3 cDNA contains an open reading frame (1524 bp) encoding 507 amino acids. The putative amino acid identity of pig LAG-3 with those of the human, rat, and mouse is 78%, 66%, and 67%, respectively. Pig Lag3 mRNA transcript was mainly expressed in lymphoid tissues, and was inducible upon stimulation of phytohemagglutinin (PHA). Next, we developed mouse antiserum against the extracellular domain (D1 and D2 domains) of pig LAG-3. Pig LAG-3 is detected as an approximately 67 kDa of a single species by Western blot analysis. Moreover, flow cytometry analysis confirmed that LAG-3 on pig T cells (CD3+ cells) is inducible upon PHA stimulation. These results indicated that the structure and expression pattern of pig LAG-3 are conserved among mammalian species.
Bibliographical noteFunding Information:
This study was supported by Technology Development Program for Agriculture and Forestry (project number: 108089-3), Ministry for Agriculture, Forestry and Fisheries, Republic of Korea (to T. Chun) and a grant from Biogreen 21 project (project number: 20070401-034-026-007-06), Rural Development Administration, Republic of Korea (to H.-S. Kang).
- Immune homeostasis
- Lymphocyte activation gene-3
- T cell activation
ASJC Scopus subject areas