Molecular cloning and expression of Turkey inhibin-α and -βa subunits

J. Ahn; S. You; H. Kim; D. N. Foster; M. E. El Halawani

doi:10.1093/ps/80.12.1690

Molecular cloning and expression of Turkey inhibin-α and -βa subunits

J. Ahn, S. You, H. Kim, D. N. Foster, M. E. El Halawani

Research output: Contribution to journal › Article › peer-review

Abstract

We isolated cDNA encoding turkey inhibin-α (tINH-α) and -βA (tINH-βA) subunits from the turkey ovary using reverse transcription-polymerase chain reaction (RT-PCR). The isolated α subunit and βA subunit included the entire open reading frames encoding 329 and 424 amino acids, respectively. The amino acid sequences of mature tINH-α subunit and tINH-βA subunit (12.6 and 12.9 kDa proteins, respectively), established via DNA sequence analysis, were highly conserved between the chicken and various mammals. Northern blot analysis revealed that the transcripts of tINH-α and tINH-βA subunits were approximately 1.7 and 8.4 kb, respectively. In various stages of follicular development, tINH-α mRNA was highly expressed in small white follicles as compared to postovulatory and regressed follicles, whereas tINH-βA mRNA was predominately expressed in preovulatory F5 follicles.

Original language	English
Pages (from-to)	1690-1694
Number of pages	5
Journal	Poultry Science
Volume	80
Issue number	12
DOIs	https://doi.org/10.1093/ps/80.12.1690
Publication status	Published - 2001 Dec
Externally published	Yes

Keywords

Subunit-βa
Turkey inhibin-α

ASJC Scopus subject areas

Animal Science and Zoology

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10.1093/ps/80.12.1690

Cite this

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title = "Molecular cloning and expression of Turkey inhibin-α and -βa subunits",

abstract = "We isolated cDNA encoding turkey inhibin-α (tINH-α) and -βA (tINH-βA) subunits from the turkey ovary using reverse transcription-polymerase chain reaction (RT-PCR). The isolated α subunit and βA subunit included the entire open reading frames encoding 329 and 424 amino acids, respectively. The amino acid sequences of mature tINH-α subunit and tINH-βA subunit (12.6 and 12.9 kDa proteins, respectively), established via DNA sequence analysis, were highly conserved between the chicken and various mammals. Northern blot analysis revealed that the transcripts of tINH-α and tINH-βA subunits were approximately 1.7 and 8.4 kb, respectively. In various stages of follicular development, tINH-α mRNA was highly expressed in small white follicles as compared to postovulatory and regressed follicles, whereas tINH-βA mRNA was predominately expressed in preovulatory F5 follicles.",

keywords = "Subunit-βa, Turkey inhibin-α",

author = "J. Ahn and S. You and H. Kim and Foster, {D. N.} and {El Halawani}, {M. E.}",

year = "2001",

month = dec,

doi = "10.1093/ps/80.12.1690",

language = "English",

volume = "80",

pages = "1690--1694",

journal = "Poultry Science",

issn = "0032-5791",

publisher = "Poultry Science Association",

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TY - JOUR

T1 - Molecular cloning and expression of Turkey inhibin-α and -βa subunits

AU - Ahn, J.

AU - You, S.

AU - Kim, H.

AU - Foster, D. N.

AU - El Halawani, M. E.

PY - 2001/12

Y1 - 2001/12

N2 - We isolated cDNA encoding turkey inhibin-α (tINH-α) and -βA (tINH-βA) subunits from the turkey ovary using reverse transcription-polymerase chain reaction (RT-PCR). The isolated α subunit and βA subunit included the entire open reading frames encoding 329 and 424 amino acids, respectively. The amino acid sequences of mature tINH-α subunit and tINH-βA subunit (12.6 and 12.9 kDa proteins, respectively), established via DNA sequence analysis, were highly conserved between the chicken and various mammals. Northern blot analysis revealed that the transcripts of tINH-α and tINH-βA subunits were approximately 1.7 and 8.4 kb, respectively. In various stages of follicular development, tINH-α mRNA was highly expressed in small white follicles as compared to postovulatory and regressed follicles, whereas tINH-βA mRNA was predominately expressed in preovulatory F5 follicles.

AB - We isolated cDNA encoding turkey inhibin-α (tINH-α) and -βA (tINH-βA) subunits from the turkey ovary using reverse transcription-polymerase chain reaction (RT-PCR). The isolated α subunit and βA subunit included the entire open reading frames encoding 329 and 424 amino acids, respectively. The amino acid sequences of mature tINH-α subunit and tINH-βA subunit (12.6 and 12.9 kDa proteins, respectively), established via DNA sequence analysis, were highly conserved between the chicken and various mammals. Northern blot analysis revealed that the transcripts of tINH-α and tINH-βA subunits were approximately 1.7 and 8.4 kb, respectively. In various stages of follicular development, tINH-α mRNA was highly expressed in small white follicles as compared to postovulatory and regressed follicles, whereas tINH-βA mRNA was predominately expressed in preovulatory F5 follicles.

KW - Subunit-βa

KW - Turkey inhibin-α

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U2 - 10.1093/ps/80.12.1690

DO - 10.1093/ps/80.12.1690

M3 - Article

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AN - SCOPUS:0035651112

SN - 0032-5791

VL - 80

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EP - 1694

JO - Poultry Science

JF - Poultry Science

IS - 12

ER -

Molecular cloning and expression of Turkey inhibin-α and -βa subunits

Abstract

Keywords

ASJC Scopus subject areas

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