Molecular cloning of the Ecotin gene in Escherichia coli

Hae Ryun Lee; Jae Hong Seol; Ok Mae Kim; Cheol Soon Lee; Se Won Suh; Yeong Man Hong; Keiji Tanaka; Akira Ichihara; Doo Bong Ha; Cin Ha Chung

doi:10.1016/0014-5793(91)80014-T

Molecular cloning of the Ecotin gene in Escherichia coli

Hae Ryun Lee, Jae Hong Seol, Ok Mae Kim, Cheol Soon Lee, Se Won Suh, Yeong Man Hong, Keiji Tanaka, Akira Ichihara, Doo Bong Ha, Cin Ha Chung

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

The nucleotide sequence of a 876 bp region in E. coli chromosome that encodes Ecotin was determined. The proposed coding sequence for Ecotin is 486 nucleotides long, which would encode a protein consisting of 162 amino acids with a calculated molecular weight of 18 192 Da. The deduced primary sequence of Ecotin includes a 20-residue signal sequence, cleavage of which would give rise to a mature protein with a molecular weight of 16 099 Da. Ecotin does not contain any consensus reactive site sequences of known serine protease inhibitor families, suggesting that Ecotin is a novel inhibitor.

Original language	English
Pages (from-to)	53-56
Number of pages	4
Journal	FEBS Letters
Volume	287
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(91)80014-T
Publication status	Published - 1991 Aug 5
Externally published	Yes

Keywords

Ecotin
Protease inhibitor
Signal sequence
eti gene

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(91)80014-T

Cite this

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title = "Molecular cloning of the Ecotin gene in Escherichia coli",

abstract = "The nucleotide sequence of a 876 bp region in E. coli chromosome that encodes Ecotin was determined. The proposed coding sequence for Ecotin is 486 nucleotides long, which would encode a protein consisting of 162 amino acids with a calculated molecular weight of 18 192 Da. The deduced primary sequence of Ecotin includes a 20-residue signal sequence, cleavage of which would give rise to a mature protein with a molecular weight of 16 099 Da. Ecotin does not contain any consensus reactive site sequences of known serine protease inhibitor families, suggesting that Ecotin is a novel inhibitor.",

keywords = "Ecotin, Protease inhibitor, Signal sequence, eti gene",

author = "Lee, {Hae Ryun} and Seol, {Jae Hong} and Kim, {Ok Mae} and Lee, {Cheol Soon} and Suh, {Se Won} and Hong, {Yeong Man} and Keiji Tanaka and Akira Ichihara and Ha, {Doo Bong} and Chung, {Cin Ha}",

note = "Funding Information: Ackno~~,/e~~enzents:T his work was supported by grants from The klinistry of Education and Korea Science and Engineering Foundation through SRC for Cell Differentiation.",

year = "1991",

month = aug,

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doi = "10.1016/0014-5793(91)80014-T",

language = "English",

volume = "287",

pages = "53--56",

journal = "FEBS Letters",

issn = "0014-5793",

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number = "1-2",

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TY - JOUR

T1 - Molecular cloning of the Ecotin gene in Escherichia coli

AU - Lee, Hae Ryun

AU - Seol, Jae Hong

AU - Kim, Ok Mae

AU - Lee, Cheol Soon

AU - Suh, Se Won

AU - Hong, Yeong Man

AU - Tanaka, Keiji

AU - Ichihara, Akira

AU - Ha, Doo Bong

AU - Chung, Cin Ha

N1 - Funding Information: Ackno~~,/e~~enzents:T his work was supported by grants from The klinistry of Education and Korea Science and Engineering Foundation through SRC for Cell Differentiation.

PY - 1991/8/5

Y1 - 1991/8/5

N2 - The nucleotide sequence of a 876 bp region in E. coli chromosome that encodes Ecotin was determined. The proposed coding sequence for Ecotin is 486 nucleotides long, which would encode a protein consisting of 162 amino acids with a calculated molecular weight of 18 192 Da. The deduced primary sequence of Ecotin includes a 20-residue signal sequence, cleavage of which would give rise to a mature protein with a molecular weight of 16 099 Da. Ecotin does not contain any consensus reactive site sequences of known serine protease inhibitor families, suggesting that Ecotin is a novel inhibitor.

AB - The nucleotide sequence of a 876 bp region in E. coli chromosome that encodes Ecotin was determined. The proposed coding sequence for Ecotin is 486 nucleotides long, which would encode a protein consisting of 162 amino acids with a calculated molecular weight of 18 192 Da. The deduced primary sequence of Ecotin includes a 20-residue signal sequence, cleavage of which would give rise to a mature protein with a molecular weight of 16 099 Da. Ecotin does not contain any consensus reactive site sequences of known serine protease inhibitor families, suggesting that Ecotin is a novel inhibitor.

KW - Ecotin

KW - Protease inhibitor

KW - Signal sequence

KW - eti gene

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U2 - 10.1016/0014-5793(91)80014-T

DO - 10.1016/0014-5793(91)80014-T

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AN - SCOPUS:0025776659

SN - 0014-5793

VL - 287

SP - 53

EP - 56

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Molecular cloning of the Ecotin gene in Escherichia coli

Abstract

Keywords

ASJC Scopus subject areas

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