Molecular cloning of the Ecotin gene in Escherichia coli

Hae Ryun Lee, Jae Hong Seol, Ok Mae Kim, Cheol Soon Lee, Se Won Suh, Yeong Man Hong, Keiji Tanaka, Akira Ichihara, Doo Bong Ha, Cin Ha Chung

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


The nucleotide sequence of a 876 bp region in E. coli chromosome that encodes Ecotin was determined. The proposed coding sequence for Ecotin is 486 nucleotides long, which would encode a protein consisting of 162 amino acids with a calculated molecular weight of 18 192 Da. The deduced primary sequence of Ecotin includes a 20-residue signal sequence, cleavage of which would give rise to a mature protein with a molecular weight of 16 099 Da. Ecotin does not contain any consensus reactive site sequences of known serine protease inhibitor families, suggesting that Ecotin is a novel inhibitor.

Original languageEnglish
Pages (from-to)53-56
Number of pages4
JournalFEBS Letters
Issue number1-2
Publication statusPublished - 1991 Aug 5
Externally publishedYes

Bibliographical note

Funding Information:
Ackno~~,/e~~enzents:T his work was supported by grants from The klinistry of Education and Korea Science and Engineering Foundation through SRC for Cell Differentiation.


  • Ecotin
  • Protease inhibitor
  • Signal sequence
  • eti gene

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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