Molecular cytogenetic analysis of the monoblastic cell line U937: Karyotype clarification by G-banding, whole chromosome painting, microdissection and reverse painting, and comparative genomic hybridization

Ji Yun Lee; Chul Hoon Lee; Sung Han Shim; Han Kyu Seo; Jee Hong Kyhm; Sechin Cho; Youl Hee Cho

doi:10.1016/S0165-4608(02)00565-4

Molecular cytogenetic analysis of the monoblastic cell line U937: Karyotype clarification by G-banding, whole chromosome painting, microdissection and reverse painting, and comparative genomic hybridization

Ji Yun Lee, Chul Hoon Lee, Sung Han Shim, Han Kyu Seo, Jee Hong Kyhm, Sechin Cho, Youl Hee Cho

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Previous reports on the analysis of the human monoblastic cell line U937 had described several sublines containing unidentified rearrangements and marker chromosomes. In order to determine the true nature of the rearrangements, conventional banding analysis was carried out with various combinations of molecular cytogenetic techniques: comparative genomic hybridization, fluorescence in situ hybridization (FISH) with whole chromosome painting probes, and microdissection and reverse painting FISH. The origins of the marker chromosomes were identified and the composite karyotype is described.

Original language	English
Pages (from-to)	124-132
Number of pages	9
Journal	Cancer Genetics and Cytogenetics
Volume	137
Issue number	2
DOIs	https://doi.org/10.1016/S0165-4608(02)00565-4
Publication status	Published - 2002 Sept
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported by The Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (HMP-00-CH-05-0005).

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Lee, J. Y., Lee, C. H., Shim, S. H., Seo, H. K., Kyhm, J. H., Cho, S., & Cho, Y. H. (2002). Molecular cytogenetic analysis of the monoblastic cell line U937: Karyotype clarification by G-banding, whole chromosome painting, microdissection and reverse painting, and comparative genomic hybridization. Cancer Genetics and Cytogenetics, 137(2), 124-132. https://doi.org/10.1016/S0165-4608(02)00565-4

Molecular cytogenetic analysis of the monoblastic cell line U937: Karyotype clarification by G-banding, whole chromosome painting, microdissection and reverse painting, and comparative genomic hybridization. / Lee, Ji Yun; Lee, Chul Hoon; Shim, Sung Han et al.
In: Cancer Genetics and Cytogenetics, Vol. 137, No. 2, 09.2002, p. 124-132.

Research output: Contribution to journal › Article › peer-review

Lee, JY, Lee, CH, Shim, SH, Seo, HK, Kyhm, JH, Cho, S & Cho, YH 2002, 'Molecular cytogenetic analysis of the monoblastic cell line U937: Karyotype clarification by G-banding, whole chromosome painting, microdissection and reverse painting, and comparative genomic hybridization', Cancer Genetics and Cytogenetics, vol. 137, no. 2, pp. 124-132. https://doi.org/10.1016/S0165-4608(02)00565-4

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abstract = "Previous reports on the analysis of the human monoblastic cell line U937 had described several sublines containing unidentified rearrangements and marker chromosomes. In order to determine the true nature of the rearrangements, conventional banding analysis was carried out with various combinations of molecular cytogenetic techniques: comparative genomic hybridization, fluorescence in situ hybridization (FISH) with whole chromosome painting probes, and microdissection and reverse painting FISH. The origins of the marker chromosomes were identified and the composite karyotype is described.",

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Molecular cytogenetic analysis of the monoblastic cell line U937: Karyotype clarification by G-banding, whole chromosome painting, microdissection and reverse painting, and comparative genomic hybridization

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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