Molecular dissection of the interaction between hBLT2 and the G protein alpha subunits

Krishna Moorthy Vukoti; Won Kyu Lee; Ho Jun Kim; Young Kim Ick; Gyeong Yang Eun; Cheolju Lee; Gyu Yu Yeon

doi:10.5012/bkcs.2007.28.6.1005

Molecular dissection of the interaction between hBLT2 and the G protein alpha subunits

Krishna Moorthy Vukoti, Won Kyu Lee, Ho Jun Kim, Young Kim Ick, Gyeong Yang Eun, Cheolju Lee, Gyu Yu Yeon

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Leukotriene B4 (LTB4) is a potent chemoattractant for leukocytes and considered to be an inflammatory mediator. Human BLT2 (hBLT2) is a low-affinity G-protein coupled receptor for LTB4 and mediates pertussis toxin-sensitive chemotactic cell movement. Here, we dissected the interaction between hBLT2 and G-protein alpha subunits using GST fusion proteins containing intracellular regions of hBLT2 and various G α protein including G αi1, G αi2, G αi3, G αs1, G αo1, and G αz. Among the tested G α subunits, G αi3 showed the highest binding to the third intracellular loop region of hBLT2 with a dissociation constant (K_D) of 5.0 × 10^-6 M. These results suggest that G αi3 has the highest affinity to hBLT2, and the third intracellular loop region of hBLT2 is the major component for the interaction with G αi3.

Original language	English
Pages (from-to)	1005-1009
Number of pages	5
Journal	Bulletin of the Korean Chemical Society
Volume	28
Issue number	6
DOIs	https://doi.org/10.5012/bkcs.2007.28.6.1005
Publication status	Published - 2007 Jun 20

Keywords

G-protein
GPCR-G protein interaction
Intracellular loop
Leukotriene B4
hBLT2

ASJC Scopus subject areas

General Chemistry

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10.5012/bkcs.2007.28.6.1005

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title = "Molecular dissection of the interaction between hBLT2 and the G protein alpha subunits",

abstract = "Leukotriene B4 (LTB4) is a potent chemoattractant for leukocytes and considered to be an inflammatory mediator. Human BLT2 (hBLT2) is a low-affinity G-protein coupled receptor for LTB4 and mediates pertussis toxin-sensitive chemotactic cell movement. Here, we dissected the interaction between hBLT2 and G-protein alpha subunits using GST fusion proteins containing intracellular regions of hBLT2 and various G α protein including G αi1, G αi2, G αi3, G αs1, G αo1, and G αz. Among the tested G α subunits, G αi3 showed the highest binding to the third intracellular loop region of hBLT2 with a dissociation constant (KD) of 5.0 × 10-6 M. These results suggest that G αi3 has the highest affinity to hBLT2, and the third intracellular loop region of hBLT2 is the major component for the interaction with G αi3.",

keywords = "G-protein, GPCR-G protein interaction, Intracellular loop, Leukotriene B4, hBLT2",

author = "Vukoti, {Krishna Moorthy} and Lee, {Won Kyu} and Kim, {Ho Jun} and Ick, {Young Kim} and Eun, {Gyeong Yang} and Cheolju Lee and Yeon, {Gyu Yu}",

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doi = "10.5012/bkcs.2007.28.6.1005",

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T1 - Molecular dissection of the interaction between hBLT2 and the G protein alpha subunits

AU - Vukoti, Krishna Moorthy

AU - Lee, Won Kyu

AU - Kim, Ho Jun

AU - Ick, Young Kim

AU - Eun, Gyeong Yang

AU - Lee, Cheolju

AU - Yeon, Gyu Yu

PY - 2007/6/20

Y1 - 2007/6/20

N2 - Leukotriene B4 (LTB4) is a potent chemoattractant for leukocytes and considered to be an inflammatory mediator. Human BLT2 (hBLT2) is a low-affinity G-protein coupled receptor for LTB4 and mediates pertussis toxin-sensitive chemotactic cell movement. Here, we dissected the interaction between hBLT2 and G-protein alpha subunits using GST fusion proteins containing intracellular regions of hBLT2 and various G α protein including G αi1, G αi2, G αi3, G αs1, G αo1, and G αz. Among the tested G α subunits, G αi3 showed the highest binding to the third intracellular loop region of hBLT2 with a dissociation constant (KD) of 5.0 × 10-6 M. These results suggest that G αi3 has the highest affinity to hBLT2, and the third intracellular loop region of hBLT2 is the major component for the interaction with G αi3.

AB - Leukotriene B4 (LTB4) is a potent chemoattractant for leukocytes and considered to be an inflammatory mediator. Human BLT2 (hBLT2) is a low-affinity G-protein coupled receptor for LTB4 and mediates pertussis toxin-sensitive chemotactic cell movement. Here, we dissected the interaction between hBLT2 and G-protein alpha subunits using GST fusion proteins containing intracellular regions of hBLT2 and various G α protein including G αi1, G αi2, G αi3, G αs1, G αo1, and G αz. Among the tested G α subunits, G αi3 showed the highest binding to the third intracellular loop region of hBLT2 with a dissociation constant (KD) of 5.0 × 10-6 M. These results suggest that G αi3 has the highest affinity to hBLT2, and the third intracellular loop region of hBLT2 is the major component for the interaction with G αi3.

KW - G-protein

KW - GPCR-G protein interaction

KW - Intracellular loop

KW - Leukotriene B4

KW - hBLT2

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M3 - Article

AN - SCOPUS:34250876289

SN - 0253-2964

VL - 28

SP - 1005

EP - 1009

JO - Bulletin of the Korean Chemical Society

JF - Bulletin of the Korean Chemical Society

IS - 6

ER -

Molecular dissection of the interaction between hBLT2 and the G protein alpha subunits

Abstract

Keywords

ASJC Scopus subject areas

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