A new chromone analogue (1) was isolated from an EtOAc-extract of Pleosporales sp. culture medium, together with five known chromones (2 - 6). The isolation workflow was guided by a Molecular Networking-based dereplication strategy. The chemical structure of the new compound was elucidated using NMR and MS spectroscopy, and the absolute configuration was established by the Mosher’s method. All isolated compounds were evaluated for their inhibitory effects on lipopolysaccharide-induced nitirc oxide production in RAW 264.7 macrophages. Compound 1 showed marginal inhibitory activity with an IC50 value of 118.7 µM.
Bibliographical noteFunding Information:
This research was supported by the Korea University and the National Research Foundation of Korea (NRF-2019R1A2C1006226 and NRF-2019R1A4A1020626) and Korea Polar Research Institute (Grant: PE20010).
© 2020, Korean Society of Pharmacognosy. All rights reserved.
- Molecular networking
- Mosher’s method
- Nitric oxide production
- Pleosporales sp.
ASJC Scopus subject areas
- Drug Discovery
- Organic Chemistry