TY - JOUR
T1 - Molecular test for vivax malaria with loop-mediated isothermal amplification method in central China
AU - Lu, Feng
AU - Gao, Qi
AU - Zhou, Huayun
AU - Cao, Jun
AU - Wang, Weimin
AU - Lim, Chae Seung
AU - Na, Sung Hun
AU - Tsuboi, Takafumi
AU - Han, Eun Taek
N1 - Funding Information:
Acknowledgments This work was supported by a National Research Foundation of Korea Grant funded by the Korean Government (2009-0075103) and the Mid-Career Researcher Program through NRF grant funded by the MEST (No. 2011-0016401) of Korea and the Asia Pacific Malaria Elimination Network Establishment Support Programme (No. 107-02), the National S & T Major Programme (No. 2008ZX10004-011), and the National Natural Science Foundation of China (No. 30872214).
PY - 2012/6
Y1 - 2012/6
N2 - Vivax malaria has the highest incidence in central China. High-throughput and cost-effective testing methods are essential for vivax malaria diagnosis in this area. Loopmediated isothermal amplification (LAMP) is an established nucleic acid amplification method, which provides a promising platform for the molecular detection of malaria parasites in development countries. This study was performed to compare the LAMP method, the nested PCR-based method, and microscopic examinations in diagnosing vivax malaria. LAMP detected vivax malaria parasites in 160 of 164 microscopically positive samples (sensitivity, 97.6%), whereas nested PCR detected Plasmodium vivax in 162 of 164 samples (sensitivity, 98.8%). No false-positive results were obtained by LAMP or nested PCR among fever-positive and healthy samples. The sensitivities and specificities of the two molecular tests were consistently high. In addition, the reproducibility of the LAMP assays using water bath, which reduced the cost substantially. LAMP method is an accurate, rapid, simple, and cost-effective method that may be useful for diagnosis in field diagnoses instead of nested PCR. This method is feasible to diagnose vivax malaria parasite in endemic areas of central China.
AB - Vivax malaria has the highest incidence in central China. High-throughput and cost-effective testing methods are essential for vivax malaria diagnosis in this area. Loopmediated isothermal amplification (LAMP) is an established nucleic acid amplification method, which provides a promising platform for the molecular detection of malaria parasites in development countries. This study was performed to compare the LAMP method, the nested PCR-based method, and microscopic examinations in diagnosing vivax malaria. LAMP detected vivax malaria parasites in 160 of 164 microscopically positive samples (sensitivity, 97.6%), whereas nested PCR detected Plasmodium vivax in 162 of 164 samples (sensitivity, 98.8%). No false-positive results were obtained by LAMP or nested PCR among fever-positive and healthy samples. The sensitivities and specificities of the two molecular tests were consistently high. In addition, the reproducibility of the LAMP assays using water bath, which reduced the cost substantially. LAMP method is an accurate, rapid, simple, and cost-effective method that may be useful for diagnosis in field diagnoses instead of nested PCR. This method is feasible to diagnose vivax malaria parasite in endemic areas of central China.
UR - http://www.scopus.com/inward/record.url?scp=84863425553&partnerID=8YFLogxK
U2 - 10.1007/s00436-011-2783-8
DO - 10.1007/s00436-011-2783-8
M3 - Article
C2 - 22200962
AN - SCOPUS:84863425553
SN - 0932-0113
VL - 110
SP - 2439
EP - 2444
JO - Parasitology Research
JF - Parasitology Research
IS - 6
ER -
