Monitoring mRNA Expression Patterns in Macrophages in Response to Two Different Strains of Probiotics

Sang Pil Choi, Si Won Park, Seok Jin Kang, Seul Ki Lim, Min Sung Kwon, Hak Jong Choi, Taehoon Chun

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    As an initial study to elucidate the molecular mechanism of how probiotics modulate macrophage activity, we monitored mRNA expression patterns in peritoneal macrophages (PMs) treated with two different strains of probiotics. After treatment with either Weissella cibaria WIKIM28 or Latilactobacillus sakei WIKIM50, total RNAs from PMs were isolated and subjected into gene chip analyses. As controls, mRNAs from vehicle (phosphate-buffered saline, PBS)-treated PMs were also subjected to gene chip analysis. Compared to vehicle (PBS)-treated PMs, WIKIM28-treated and WIKIM50-treated PMs exhibited a total of 889 and 432 differentially expressed genes with expression differences of at least 4 folds, respectively. Compared to WIKIM28-treated PMs, WIKIM50-treated PMs showed 25 up-regulated genes and 21 down-regulated genes with expression differences of more than 2 folds. Interestingly, mRNA transcripts of M2 macrophage polarization marker such as anxa1, mafb, and sepp1 were increased in WIKIM50-treated PMs comparing to those in WIKIM28-treated PMs. Reversely, mRNA transcripts of M1 macrophage polarization marker such as hdac9, ptgs2, and socs3 were decreased in WIKIM50-treated PMs comparing to those in WIKIM28-treated PMs. In agreement with these observations, mRNA expression levels of tumor necrosis factor-α and interleukin-1α were significantly reduced in WIKIM50-treated macrophages compared to those in WIKIM28-treated macrophages. These results may indicate that probiotics can be classified as two different types depending on their ability to convert macrophages into M1 or M2 polarization.

    Original languageEnglish
    Pages (from-to)703-711
    Number of pages9
    JournalFood Science of Animal Resources
    Volume43
    Issue number4
    DOIs
    Publication statusPublished - 2023 Jul

    Bibliographical note

    Publisher Copyright:
    © Korean Society for Food Science of Animal Resources.

    Keywords

    • differentially expressed gene
    • macrophage
    • probiotics

    ASJC Scopus subject areas

    • Food Science
    • Animal Science and Zoology

    Fingerprint

    Dive into the research topics of 'Monitoring mRNA Expression Patterns in Macrophages in Response to Two Different Strains of Probiotics'. Together they form a unique fingerprint.

    Cite this