Abstract
The methanol extract from the aerial parts of Dictamnus albus was active in inhibiting monoamine oxidase (MAO) from the mouse brain. Activity-guided fractionation led to the isolation of four known coumarins, 7-(6′R- hydroxy-3′, 7′-dimethyl-2′E, 7′-octadienyloxy) coumarin (1), auraptene (2), umbelliferone (3), and xanthotoxin (4), as active compounds along with an inactive alkaloid, skimmianine (5). Compounds 1 and 2 inhibited MAO activity in a concentration-dependent manner with IC50 values of 0.7 and 1.7 μM, respectively. Compounds 1 and 2 showed a slight and potently selective inhibitory effect against MAO-B (IC50 0.5 and 0.6 μM, respectively) compared to MAO-A (IC50 1.3 and 34.6 μM, respectively). According to kinetic analyses derived by Lineweaver-Burk reciprocal plots, compounds 1 and 2 exhibited a competitive inhibition to MAO-B.
Original language | English |
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Pages (from-to) | 1119-1124 |
Number of pages | 6 |
Journal | Archives of pharmacal research |
Volume | 29 |
Issue number | 12 |
DOIs | |
Publication status | Published - 2006 Dec 31 |
Keywords
- Coumarin
- Dictamnus albus
- Monoamine oxidase inhibitor
- Rutaceae
ASJC Scopus subject areas
- Molecular Medicine
- Drug Discovery
- Organic Chemistry