TY - JOUR
T1 - Morphological analysis of spine shapes of Purkinje cell dendrites in the rat cerebellum using high-voltage electron microscopy
AU - Lee, Kea Joo
AU - Kim, Hyun
AU - Kim, Tae Sik
AU - Park, Sun-Hwa
AU - Rhyu, Im Joo
N1 - Funding Information:
The authors are grateful to Dr K. Hama for his useful comments and histological methods. We are also in debted to Dr. I.J. Weiler for her kind reading of this manuscript. This study was supported by a grant from the Korean Health 21 R&D Project, Korean Ministry of Health Welfare (HMP-00-GN-01-0002).
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004/4/8
Y1 - 2004/4/8
N2 - Morphological changes in spine shapes have been implicated as indications for physiological or pathological status. To investigate the normal distribution ratio of spine shapes of rat Purkinje cells, morphological analysis was conducted using high-voltage electron microscopy following Golgi impregnation. Spines were classified into thin, stubby, mushroom, branched, and unclassified type by their distinct morphological features. In the tertiary branches of Purkinje cell dendrites, proportions of each category were 69.11±1.38% (thin), 13.5±1.23% (stubby), 10.45±0.74% (mushroom), 2.21±0.31% (branched), and 4.73±0.52% (unclassified). These results suggest that dendritic spines of Purkinje cells may tend to cluster in defined groups by shapes implying that different spine shapes could reflect different functional roles. This classification could be applied for further study of spine plasticity in various conditions.
AB - Morphological changes in spine shapes have been implicated as indications for physiological or pathological status. To investigate the normal distribution ratio of spine shapes of rat Purkinje cells, morphological analysis was conducted using high-voltage electron microscopy following Golgi impregnation. Spines were classified into thin, stubby, mushroom, branched, and unclassified type by their distinct morphological features. In the tertiary branches of Purkinje cell dendrites, proportions of each category were 69.11±1.38% (thin), 13.5±1.23% (stubby), 10.45±0.74% (mushroom), 2.21±0.31% (branched), and 4.73±0.52% (unclassified). These results suggest that dendritic spines of Purkinje cells may tend to cluster in defined groups by shapes implying that different spine shapes could reflect different functional roles. This classification could be applied for further study of spine plasticity in various conditions.
KW - Dendritic spines
KW - Golgi impregnation
KW - High-voltage electron microscopy
KW - Purkinje cells
KW - Stereoscopic images
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U2 - 10.1016/j.neulet.2004.01.071
DO - 10.1016/j.neulet.2004.01.071
M3 - Article
C2 - 15050702
AN - SCOPUS:1842504415
SN - 0304-3940
VL - 359
SP - 21
EP - 24
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1-2
ER -