Morphological analysis of spine shapes of Purkinje cell dendrites in the rat cerebellum using high-voltage electron microscopy

Kea Joo Lee; Hyun Kim; Tae Sik Kim; Sun Hwa Park; Im Joo Rhyu

doi:10.1016/j.neulet.2004.01.071

Morphological analysis of spine shapes of Purkinje cell dendrites in the rat cerebellum using high-voltage electron microscopy

Kea Joo Lee
, Hyun Kim
, Tae Sik Kim
, Sun Hwa Park
, Im Joo Rhyu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

Morphological changes in spine shapes have been implicated as indications for physiological or pathological status. To investigate the normal distribution ratio of spine shapes of rat Purkinje cells, morphological analysis was conducted using high-voltage electron microscopy following Golgi impregnation. Spines were classified into thin, stubby, mushroom, branched, and unclassified type by their distinct morphological features. In the tertiary branches of Purkinje cell dendrites, proportions of each category were 69.11±1.38% (thin), 13.5±1.23% (stubby), 10.45±0.74% (mushroom), 2.21±0.31% (branched), and 4.73±0.52% (unclassified). These results suggest that dendritic spines of Purkinje cells may tend to cluster in defined groups by shapes implying that different spine shapes could reflect different functional roles. This classification could be applied for further study of spine plasticity in various conditions.

Original language	English
Pages (from-to)	21-24
Number of pages	4
Journal	Neuroscience Letters
Volume	359
Issue number	1-2
DOIs	https://doi.org/10.1016/j.neulet.2004.01.071
Publication status	Published - 2004 Apr 8

Bibliographical note

Funding Information:
The authors are grateful to Dr K. Hama for his useful comments and histological methods. We are also in debted to Dr. I.J. Weiler for her kind reading of this manuscript. This study was supported by a grant from the Korean Health 21 R&D Project, Korean Ministry of Health Welfare (HMP-00-GN-01-0002).

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

Keywords

Dendritic spines
Golgi impregnation
High-voltage electron microscopy
Purkinje cells
Stereoscopic images

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1016/j.neulet.2004.01.071

Cite this

@article{c9cdb519891947dc9d8eed622626ba96,

title = "Morphological analysis of spine shapes of Purkinje cell dendrites in the rat cerebellum using high-voltage electron microscopy",

abstract = "Morphological changes in spine shapes have been implicated as indications for physiological or pathological status. To investigate the normal distribution ratio of spine shapes of rat Purkinje cells, morphological analysis was conducted using high-voltage electron microscopy following Golgi impregnation. Spines were classified into thin, stubby, mushroom, branched, and unclassified type by their distinct morphological features. In the tertiary branches of Purkinje cell dendrites, proportions of each category were 69.11±1.38\% (thin), 13.5±1.23\% (stubby), 10.45±0.74\% (mushroom), 2.21±0.31\% (branched), and 4.73±0.52\% (unclassified). These results suggest that dendritic spines of Purkinje cells may tend to cluster in defined groups by shapes implying that different spine shapes could reflect different functional roles. This classification could be applied for further study of spine plasticity in various conditions.",

keywords = "Dendritic spines, Golgi impregnation, High-voltage electron microscopy, Purkinje cells, Stereoscopic images",

author = "Lee, \{Kea Joo\} and Hyun Kim and Kim, \{Tae Sik\} and Park, \{Sun Hwa\} and Rhyu, \{Im Joo\}",

note = "Funding Information: The authors are grateful to Dr K. Hama for his useful comments and histological methods. We are also in debted to Dr. I.J. Weiler for her kind reading of this manuscript. This study was supported by a grant from the Korean Health 21 R\&D Project, Korean Ministry of Health Welfare (HMP-00-GN-01-0002). Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2004",

month = apr,

day = "8",

doi = "10.1016/j.neulet.2004.01.071",

language = "English",

volume = "359",

pages = "21--24",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "1-2",

}

TY - JOUR

T1 - Morphological analysis of spine shapes of Purkinje cell dendrites in the rat cerebellum using high-voltage electron microscopy

AU - Lee, Kea Joo

AU - Kim, Hyun

AU - Kim, Tae Sik

AU - Park, Sun Hwa

AU - Rhyu, Im Joo

N1 - Funding Information: The authors are grateful to Dr K. Hama for his useful comments and histological methods. We are also in debted to Dr. I.J. Weiler for her kind reading of this manuscript. This study was supported by a grant from the Korean Health 21 R&D Project, Korean Ministry of Health Welfare (HMP-00-GN-01-0002). Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2004/4/8

Y1 - 2004/4/8

N2 - Morphological changes in spine shapes have been implicated as indications for physiological or pathological status. To investigate the normal distribution ratio of spine shapes of rat Purkinje cells, morphological analysis was conducted using high-voltage electron microscopy following Golgi impregnation. Spines were classified into thin, stubby, mushroom, branched, and unclassified type by their distinct morphological features. In the tertiary branches of Purkinje cell dendrites, proportions of each category were 69.11±1.38% (thin), 13.5±1.23% (stubby), 10.45±0.74% (mushroom), 2.21±0.31% (branched), and 4.73±0.52% (unclassified). These results suggest that dendritic spines of Purkinje cells may tend to cluster in defined groups by shapes implying that different spine shapes could reflect different functional roles. This classification could be applied for further study of spine plasticity in various conditions.

AB - Morphological changes in spine shapes have been implicated as indications for physiological or pathological status. To investigate the normal distribution ratio of spine shapes of rat Purkinje cells, morphological analysis was conducted using high-voltage electron microscopy following Golgi impregnation. Spines were classified into thin, stubby, mushroom, branched, and unclassified type by their distinct morphological features. In the tertiary branches of Purkinje cell dendrites, proportions of each category were 69.11±1.38% (thin), 13.5±1.23% (stubby), 10.45±0.74% (mushroom), 2.21±0.31% (branched), and 4.73±0.52% (unclassified). These results suggest that dendritic spines of Purkinje cells may tend to cluster in defined groups by shapes implying that different spine shapes could reflect different functional roles. This classification could be applied for further study of spine plasticity in various conditions.

KW - Dendritic spines

KW - Golgi impregnation

KW - High-voltage electron microscopy

KW - Purkinje cells

KW - Stereoscopic images

UR - https://www.scopus.com/pages/publications/1842504415

U2 - 10.1016/j.neulet.2004.01.071

DO - 10.1016/j.neulet.2004.01.071

M3 - Article

C2 - 15050702

AN - SCOPUS:1842504415

SN - 0304-3940

VL - 359

SP - 21

EP - 24

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1-2

ER -

Morphological analysis of spine shapes of Purkinje cell dendrites in the rat cerebellum using high-voltage electron microscopy

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this