TY - JOUR
T1 - Morphological change and cell disruption of haematococcus pluvialis cyst during high-pressure homogenization for astaxanthin recovery
AU - Praveenkumar, Ramasamy
AU - Lee, Jiye
AU - Vijayan, Durairaj
AU - Lee, Soo Youn
AU - Lee, Kyubock
AU - Sim, Sang Jun
AU - Hong, Min Eui
AU - Kim, Young Eun
AU - Oh, You Kwan
N1 - Funding Information:
This work was supported by the National Research Foundation of Korea (grant number: NRF-2019R1A2C1003463) funded by the Ministry of Science and ICT and the Research/Development Program of the Korea Institute of Energy Research (grant number: KIER-B9-2442-04). Sim, S.J., also would like to acknowledge the support of the Korea CCS R and D Center (Korea CCS 2020 Project) funded by the Ministry of Science and ICT in 2017 (grant number: KCRC-2014M1A8A1049278).
Publisher Copyright:
© 2020 by the authors.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Haematococcus pluvialis accumulates astaxanthin, which is a high-value antioxidant, during the red cyst stage of its lifecycle. The development of a rigid cell wall in the cysts hinders the recovery of astaxanthin. We investigated morphological changes and cell disruption of mature H. pluvialis cyst cells while using high-pressure homogenization for astaxanthin extraction. When treated with French-press-cell (pressure, 10,000-30,000 psi; passage, 1-3), the intact cyst cells were significantly broken or fully ruptured, releasing cytoplasmic components, thereby facilitating the separation of astaxanthin by ethyl acetate. Fluorescence microscopy observations using three different fluorescent dyes revealed that a greater degree of cell breakage caused greater external dispersion of astaxanthin, chlorophyll, lipids, proteins, and carbohydrates. The mechanical treatment resulted in a high cell disruption rate of up to 91% based on microscopic cell typing and Coulter methods. After the ethyl acetate extraction, the astaxanthin concentration significantly increased by 15.2 mg/L in proportion to the increase in cell disruption rate, which indicates that cell disruption is a critical factor for solvent-based astaxanthin recovery. Furthermore, this study recommends a synergistic combination of the fast instrumental particle-volume-distribution analysis and microscope-based morphologic phenotyping for the development of practical H. pluvialis biorefinery processes that co-produce various biological products, including lipids, proteins, carbohydrates, chlorophyll, and astaxanthin.
AB - Haematococcus pluvialis accumulates astaxanthin, which is a high-value antioxidant, during the red cyst stage of its lifecycle. The development of a rigid cell wall in the cysts hinders the recovery of astaxanthin. We investigated morphological changes and cell disruption of mature H. pluvialis cyst cells while using high-pressure homogenization for astaxanthin extraction. When treated with French-press-cell (pressure, 10,000-30,000 psi; passage, 1-3), the intact cyst cells were significantly broken or fully ruptured, releasing cytoplasmic components, thereby facilitating the separation of astaxanthin by ethyl acetate. Fluorescence microscopy observations using three different fluorescent dyes revealed that a greater degree of cell breakage caused greater external dispersion of astaxanthin, chlorophyll, lipids, proteins, and carbohydrates. The mechanical treatment resulted in a high cell disruption rate of up to 91% based on microscopic cell typing and Coulter methods. After the ethyl acetate extraction, the astaxanthin concentration significantly increased by 15.2 mg/L in proportion to the increase in cell disruption rate, which indicates that cell disruption is a critical factor for solvent-based astaxanthin recovery. Furthermore, this study recommends a synergistic combination of the fast instrumental particle-volume-distribution analysis and microscope-based morphologic phenotyping for the development of practical H. pluvialis biorefinery processes that co-produce various biological products, including lipids, proteins, carbohydrates, chlorophyll, and astaxanthin.
KW - Astaxanthin
KW - Cell disruption
KW - Cyst
KW - Haematococcus pluvialis
KW - High-pressure homogenization
UR - http://www.scopus.com/inward/record.url?scp=85081272611&partnerID=8YFLogxK
U2 - 10.3390/app10020513
DO - 10.3390/app10020513
M3 - Article
AN - SCOPUS:85081272611
SN - 2076-3417
VL - 10
JO - Applied Sciences (Switzerland)
JF - Applied Sciences (Switzerland)
IS - 2
M1 - 513
ER -