Multi-layered enzyme coating on highly conductive magnetic biochar nanoparticles for bisphenol A sensing in water

Lingzhi He, Yuesuo Yang, Jungbae Kim, Lan Yao, Xinwei Dong, Taihua Li, Yunxian Piao

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)


This work introduced stable immobilization of tyrosinase (TYR) enzymes onto the highly conductive magnetic biochar nanoparticles with carboxyl functionality to construct sensitive and reusable electrochemical biosensor for bisphenol A (BPA) detection. The newly synthesized biochar nanoparticles (BCNPs) from biomass had outstanding electrochemical activity, after functionalization with carboxylic functional groups and magnetic nanoparticles (Mag-BCNPs-COOH), it was used for multi-layered TYR immobilization with covalent attachment, precipitation and cross-linking method (ML-TYR/Mag-BCNPs-COOH). The BPA sensing system was simply constructed by directly attaching the biocatalyst of ML-TYR/Mag-BCNPs-COOH onto a glassy carbon electrode with magnet force. Owning to great properties of the ML-TYR/Mag-BCNPs-COOH, such as high stability, biocatalytic activity and conductivity, the BPA sensing signal was significantly enhanced. And the lowest detection limit of 2.78 nM with linear ranges from 0.01 to 1.01 μM was achieved. The modified electrode could be reused at least eight cycles without reducing the sensing signal, and could be used for the real environmental water detection.

Original languageEnglish
Article number123276
JournalChemical Engineering Journal
Publication statusPublished - 2020 Mar 15

Bibliographical note

Funding Information:
This work was funded by the open fund from the Key Lab of Eco-restoration of Regional Contaminated Environment ( Shenyang University ), Ministry of Education (2019_ERRCE_K2), and the National Natural Science Foundation of China (51809111).

Publisher Copyright:
© 2019 Elsevier B.V.


  • Biosensor
  • Bisphenol A
  • Conductive biochar nanoparticles
  • Tyrosinase

ASJC Scopus subject areas

  • General Chemistry
  • Environmental Chemistry
  • General Chemical Engineering
  • Industrial and Manufacturing Engineering


Dive into the research topics of 'Multi-layered enzyme coating on highly conductive magnetic biochar nanoparticles for bisphenol A sensing in water'. Together they form a unique fingerprint.

Cite this